LOCUS SIU00053.1 425 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) protein. ACCESSION LT708304-1466 PROTEIN_ID SIU00053.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ribA2" /locus_tag="BQ2027_MB1450" /note="Mb1450, ribA2, len: 425 aa. Equivalent to Rv1415, len: 425 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 425 aa overlap). Probable ribA2, Riboflavin biosynthesis protein (EC 3.5.4.25), similar to many e.g. GCH2_BACSU|P17620 from Bacillus subtilis (398 aa), FASTA scores: opt: 1388, E(): 0, (55.4% identity in 399 aa overlap). Also similar to second Mycobacterium tuberculosis gtp cyclohydrolase Rv1940|ribA1 (353 aa). IN THE N-TERMINAL SECTION; BELONGS TO THE DHBP SYNTHASE FAMILY. IN THE C-TERMINAL SECTION; BELONGS TO THE GTP CYCLOHYDROLASE II FAMILY. Protein product from Mb1450 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1450 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A5V1" /db_xref="InterPro:IPR000422" /db_xref="InterPro:IPR000926" /db_xref="InterPro:IPR016299" /db_xref="InterPro:IPR017945" /db_xref="InterPro:IPR032677" /db_xref="InterPro:IPR036144" /db_xref="UniProtKB/Swiss-Prot:P0A5V1" BEGIN 1 MTRLDSVERA VADIAAGKAV IVIDDEDREN EGDLIFAAEK ATPEMVAFMV RYTSGYLCVP 61 LDGAICDRLG LLPMYAVNQD KHGTAYTVTV DARNGIGTGI SASDRATTMR LLADPTSVAD 121 DFTRPGHVVP LRAKDGGVLR RPGHTEAAVD LARMAGLQPA GAICEIVSQK DEGSMAHTDE 181 LRVFADEHGL ALITIADLIE WRRKHEKHIE RVAEARIPTR HGEFRAIGYT SIYEDVEHVA 241 LVRGEIAGPN ADGDDVLVRV HSECLTGDVF GSRRCDCGPQ LDAALAMVAR EGRGVVLYMR 301 GHEGRGIGLM HKLQAYQLQD AGADTVDANL KLGLPADARD YGIGAQILVD LGVRSMRLLT 361 NNPAKRVGLD GYGLHIIERV PLPVRANAEN IRYLMTKRDK LGHDLAGLDD FHESVHLPGE 421 FGGAL //