LOCUS SIU00030.1 403 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE S-ADENOSYLMETHIONINE SYNTHETASE METK (MAT) (AdoMet synthetase) (Methionine adenosyltransferase) protein. ACCESSION LT708304-1443 PROTEIN_ID SIU00030.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="metK" /locus_tag="BQ2027_MB1427" /note="Mb1427, metK, len: 403 aa. Equivalent to Rv1392, len: 403 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 403 aa overlap). Probable metK, S-adenosylmethionine synthetase (EC 2.5.1.6), similar to many e.g. METK_STAAU|P50307 Staphylococcus aureus (397 aa), FASTA scores: opt: 1484, E(): 0, (58.0% identity in 400 aa overlap). Contains PS00376 S-adenosylmethionine synthetase signature 1, PS00377 S-adenosylmethionine synthetase signature 2. BELONGS TO THE ADOMET SYNTHETASE FAMILY. Protein product from Mb1427 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1427 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:Q7U051" /db_xref="InterPro:IPR002133" /db_xref="InterPro:IPR022628" /db_xref="InterPro:IPR022629" /db_xref="InterPro:IPR022630" /db_xref="InterPro:IPR022631" /db_xref="InterPro:IPR022636" /db_xref="UniProtKB/Swiss-Prot:Q7U051" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSEKGRLFTS ESVTEGHPDK ICDAISDSVL DALLAADPRS RVAVETLVTT GQVHVVGEVA 61 TSAKEAFADI TNTVRARILE IGYDSSDKGF DGATCGVNIG IGAQSPDIAQ GVDTAHEARV 121 EGAADPLDSQ GAGDQGLMFG YAINATPELM PLPIALAHRL SRRLTEVRKN GVLPYLRPDG 181 KTQVTIAYED NVPVRLDTVV ISTQHAADID LEKTLDPDIR EKVLNTVLDD LAHETLDAST 241 VRVLVNPTGK FVLGGPMGDA GLTGRKIIVD TYGGWARHGG GAFSGKDPSK VDRSAAYAMR 301 WVAKNVVAAG LAERVEVQVA YAIGKAAPVG LFVETFGTET EDPVKIEKAI GEVFDLRPGA 361 IIRDLNLLRP IYAPTAAYGH FGRTDVELPW EQLDKVDDLK RAI //