LOCUS       SIU00030.1               403 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            S-ADENOSYLMETHIONINE SYNTHETASE METK (MAT) (AdoMet synthetase)
            (Methionine adenosyltransferase) protein.
ACCESSION   LT708304-1443
PROTEIN_ID  SIU00030.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="metK"
                     /locus_tag="BQ2027_MB1427"
                     /note="Mb1427, metK, len: 403 aa. Equivalent to Rv1392,
                     len: 403 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 403 aa overlap). Probable metK,
                     S-adenosylmethionine synthetase (EC 2.5.1.6), similar to
                     many e.g. METK_STAAU|P50307 Staphylococcus aureus (397
                     aa), FASTA scores: opt: 1484, E(): 0, (58.0% identity in
                     400 aa overlap). Contains PS00376 S-adenosylmethionine
                     synthetase signature 1, PS00377 S-adenosylmethionine
                     synthetase signature 2. BELONGS TO THE ADOMET SYNTHETASE
                     FAMILY. Protein product from Mb1427 detected using shotgun
                     mass spectrometry and SWATH mass spectrometry. Mb1427
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:Q7U051"
                     /db_xref="InterPro:IPR002133"
                     /db_xref="InterPro:IPR022628"
                     /db_xref="InterPro:IPR022629"
                     /db_xref="InterPro:IPR022630"
                     /db_xref="InterPro:IPR022631"
                     /db_xref="InterPro:IPR022636"
                     /db_xref="UniProtKB/Swiss-Prot:Q7U051"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MSEKGRLFTS ESVTEGHPDK ICDAISDSVL DALLAADPRS RVAVETLVTT GQVHVVGEVA
       61 TSAKEAFADI TNTVRARILE IGYDSSDKGF DGATCGVNIG IGAQSPDIAQ GVDTAHEARV
      121 EGAADPLDSQ GAGDQGLMFG YAINATPELM PLPIALAHRL SRRLTEVRKN GVLPYLRPDG
      181 KTQVTIAYED NVPVRLDTVV ISTQHAADID LEKTLDPDIR EKVLNTVLDD LAHETLDAST
      241 VRVLVNPTGK FVLGGPMGDA GLTGRKIIVD TYGGWARHGG GAFSGKDPSK VDRSAAYAMR
      301 WVAKNVVAAG LAERVEVQVA YAIGKAAPVG LFVETFGTET EDPVKIEKAI GEVFDLRPGA
      361 IIRDLNLLRP IYAPTAAYGH FGRTDVELPW EQLDKVDDLK RAI
//