LOCUS SIU00027.1 208 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE GUANYLATE KINASE GMK protein. ACCESSION LT708304-1440 PROTEIN_ID SIU00027.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="gmk" /locus_tag="BQ2027_MB1424" /note="Mb1424, gmk, len: 208 aa. Equivalent to Rv1389, len: 208 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 208 aa overlap). Probable gmk, guanylate kinase (EC 2.7.4.8), similar to e.g. KGUA_ECOLI|P24234 guanylate kinase from Escherichia coli (207 aa), FASTA scores: opt: 424, E(): 6.6e-20, (35.9% identity in 184 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00856 Guanylate kinase signature. BELONGS TO THE GUANYLATE KINASE FAMILY. Protein product from Mb1424 detected using SWATH mass spectrometry. Mb1424 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A5I5" /db_xref="InterPro:IPR008144" /db_xref="InterPro:IPR008145" /db_xref="InterPro:IPR017665" /db_xref="InterPro:IPR020590" /db_xref="InterPro:IPR027417" /db_xref="UniProtKB/Swiss-Prot:P0A5I5" BEGIN 1 MSVGEGPDTK PTARGQPAAV GRVVVLSGPS AVGKSTVVRC LRERIPNLHF SVSATTRAPR 61 PGEVDGVDYH FIDPTRFQQL IDQGELLEWA EIHGGLHRSG TLAQPVRAAA ATGVPVLIEV 121 DLAGARAIKK TMPEAVTVFL APPSWQDLQA RLIGRGTETA DVIQRRLDTA RIELAAQGDF 181 DKVVVNRRLE SACAELVSLL VGTAPGSP //