LOCUS SIT99965.1 701 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE GLUCANASE GLGE protein. ACCESSION LT708304-1377 PROTEIN_ID SIT99965.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="glgE" /locus_tag="BQ2027_MB1362C" /note="Mb1362c, glgE, len: 701 aa. Equivalent to Rv1327c, len: 701 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 701 aa overlap). Probable glgE, glucanase, similar to AF172946|AF172946_2 putative glucanase GlgE from Mycobacterium smegmatis (697 aa), FASTA scores: opt: 3816, E(): 0, (78.5% identity in 692 aa overlap). Similar to putative alpha-amylases e.g. Q9L1K2 Streptomyces coelicolor (675 aa), FASTA scores: opt: 2243, E(): 7.4e-132, (54.2% identity in 684 aa overlap). Start changed since original submission (-36) based on similarity to GlgE of Mycobacterium smegmatis; previous start at position 1494531. Protein product from Mb1362c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1362c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P63532" /db_xref="InterPro:IPR006047" /db_xref="InterPro:IPR013780" /db_xref="InterPro:IPR013783" /db_xref="InterPro:IPR017853" /db_xref="InterPro:IPR021828" /db_xref="InterPro:IPR026585" /db_xref="UniProtKB/Swiss-Prot:P63532" BEGIN 1 MSGRAIGTET EWWVPGRVEI DDVAPVVSCG VYPAKAVVGE VVPVSAAVWR EGHEAVAATL 61 VVRYLGVRYP HLTDRPRARV LPTPSEPQQR VKPLLIPMTS GQEPFVFHGQ FTPDRVGLWT 121 FRVDGWGDPI HTWRHGLIAK LDAGQGETEL SNDLLVGAVL LERAATGVPR GLRDPLLAAA 181 AALRTPGDPV TRTALALTPE IEELLADYPL RDLVTRGEQF GVWVDRPLAR FGAWYEMFPR 241 STGGWDDDGN PVHGTFATAA AELPRIAGMG FDVVYLPPIH PIGKVHRKGR NNSPTAAPTD 301 VGSPWAIGSD EGGHDTVHPS LGTIDDFDDF VSAARDLGME VALDLALQCA PDHPWAREHR 361 QWFTELPDGT IAYAENPPKK YQDIYPLNFD NDPEGLYDEV LRVVQHWVNH GVKFFRVDNP 421 HTKPPNFWAW LIAQVKTVDP DVLFLSEAFT PPARQYGLAK LGFTQSYSYF TWRTTKWELT 481 EFGNQIAELA DYRRPNLFVN TPDILHAVLQ HNGPGMFAIR AVLAATMSPA WGMYCGYELF 541 EHRAVREGSE EYLDSEKYEL RPRDFASALD QGRSLQPFIT RLNIIRRLHP AFQQLRTIHF 601 HHVDNDALLA YSKFDPATGD CVLVVVTLNA FGPEEATLWL DMAALGMEDY DRFWVRDEIT 661 GEEYQWGQAN YIRIDPARAV AHIINMPAVP YESRNTLLRR R //