LOCUS       SIT99965.1               701 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            GLUCANASE GLGE protein.
ACCESSION   LT708304-1377
PROTEIN_ID  SIT99965.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="glgE"
                     /locus_tag="BQ2027_MB1362C"
                     /note="Mb1362c, glgE, len: 701 aa. Equivalent to Rv1327c,
                     len: 701 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 701 aa overlap). Probable glgE,
                     glucanase, similar to AF172946|AF172946_2 putative
                     glucanase GlgE from Mycobacterium smegmatis (697 aa),
                     FASTA scores: opt: 3816, E(): 0, (78.5% identity in 692 aa
                     overlap). Similar to putative alpha-amylases e.g. Q9L1K2
                     Streptomyces coelicolor (675 aa), FASTA scores: opt: 2243,
                     E(): 7.4e-132, (54.2% identity in 684 aa overlap). Start
                     changed since original submission (-36) based on
                     similarity to GlgE of Mycobacterium smegmatis; previous
                     start at position 1494531. Protein product from Mb1362c
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb1362c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P63532"
                     /db_xref="InterPro:IPR006047"
                     /db_xref="InterPro:IPR013780"
                     /db_xref="InterPro:IPR013783"
                     /db_xref="InterPro:IPR017853"
                     /db_xref="InterPro:IPR021828"
                     /db_xref="InterPro:IPR026585"
                     /db_xref="UniProtKB/Swiss-Prot:P63532"
BEGIN
        1 MSGRAIGTET EWWVPGRVEI DDVAPVVSCG VYPAKAVVGE VVPVSAAVWR EGHEAVAATL
       61 VVRYLGVRYP HLTDRPRARV LPTPSEPQQR VKPLLIPMTS GQEPFVFHGQ FTPDRVGLWT
      121 FRVDGWGDPI HTWRHGLIAK LDAGQGETEL SNDLLVGAVL LERAATGVPR GLRDPLLAAA
      181 AALRTPGDPV TRTALALTPE IEELLADYPL RDLVTRGEQF GVWVDRPLAR FGAWYEMFPR
      241 STGGWDDDGN PVHGTFATAA AELPRIAGMG FDVVYLPPIH PIGKVHRKGR NNSPTAAPTD
      301 VGSPWAIGSD EGGHDTVHPS LGTIDDFDDF VSAARDLGME VALDLALQCA PDHPWAREHR
      361 QWFTELPDGT IAYAENPPKK YQDIYPLNFD NDPEGLYDEV LRVVQHWVNH GVKFFRVDNP
      421 HTKPPNFWAW LIAQVKTVDP DVLFLSEAFT PPARQYGLAK LGFTQSYSYF TWRTTKWELT
      481 EFGNQIAELA DYRRPNLFVN TPDILHAVLQ HNGPGMFAIR AVLAATMSPA WGMYCGYELF
      541 EHRAVREGSE EYLDSEKYEL RPRDFASALD QGRSLQPFIT RLNIIRRLHP AFQQLRTIHF
      601 HHVDNDALLA YSKFDPATGD CVLVVVTLNA FGPEEATLWL DMAALGMEDY DRFWVRDEIT
      661 GEEYQWGQAN YIRIDPARAV AHIINMPAVP YESRNTLLRR R
//