LOCUS       SIT99961.1               389 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ACETYL-COA ACETYLTRANSFERASE FADA4 (ACETOACETYL-COA THIOLASE)
            protein.
ACCESSION   LT708304-1373
PROTEIN_ID  SIT99961.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fadA4"
                     /locus_tag="BQ2027_MB1358"
                     /note="Mb1358, fadA4, len: 389 aa. Equivalent to Rv1323,
                     len: 389 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 389 aa overlap). Probable fadA4,
                     acetyl-CoA acetyltransferase (EC 2.3.1.9), equivalent to
                     THIL_MYCLE|P46707 possible acetyl-CoA C-acetyltransferase
                     from Mycobacterium leprae (393 aa), FASTA scores: opt:
                     2218, E(): 0, (87.0% identity in 392 aa overlap). Also
                     highly similar to others e.g. CAB70629.1|AL137242 probable
                     acetoacetyl-coA thiolase from Streptomyces coelicolor (401
                     aa); T51772 acetyl-CoA C-acetyltransferase (EC 2.3.1.9)
                     [validated] from Alcaligenes latus (392 aa); etc. Some
                     homologies indicate ATA start codon. Contains PS00098
                     Thiolases acyl-enzyme intermediate signature, PS00737
                     Thiolases signature 2, and PS00099 Thiolases active site.
                     BELONGS TO THE THIOLASE FAMILY. Protein product from
                     Mb1358 detected using shotgun mass spectrometry and SWATH
                     mass spectrometry. Mb1358 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P66927"
                     /db_xref="InterPro:IPR002155"
                     /db_xref="InterPro:IPR016039"
                     /db_xref="InterPro:IPR020610"
                     /db_xref="InterPro:IPR020613"
                     /db_xref="InterPro:IPR020615"
                     /db_xref="InterPro:IPR020616"
                     /db_xref="InterPro:IPR020617"
                     /db_xref="UniProtKB/Swiss-Prot:P66927"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MIVAGARTPI GKLMGSLKDF SASELGAIAI KGALEKANVP ASLVEYVIMG QVLTAGAGQM
       61 PARQAAVAAG IGWDVPALTI NKMCLSGIDA IALADQLIRA REFDVVVAGG QESMTKAPHL
      121 LMNSRSGYKY GDVTVLDHMA YDGLHDVFTD QPMGALTEQR NDVDMFTRSE QDEYAAASHQ
      181 KAAAAWKDGV FADEVIPVNI PQRTGDPLQF TEDEGIRANT TAAALAGLKP AFRGDGTITA
      241 GSASQISDGA AAVVVMNQEK AQELGLTWLA EIGAHGVVAG PDSTLQSQPA NAINKALDRE
      301 GISVDQLDVV EINEAFAAVA LASIRELGLN PQIVNVNGGA IAVGHPLGMS GTRITLHAAL
      361 QLARRGSGVG VAALCGAGGQ GDALILRAG
//