LOCUS SIT99957.1 567 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE ADENYLATE CYCLASE (ATP PYROPHOSPHATE-LYASE) (ADENYLYL CYCLASE) protein. ACCESSION LT708304-1369 PROTEIN_ID SIT99957.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1354C" /note="Mb1354c, -, len: 567 aa. Equivalent to Rv1320c, len: 567 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 567 aa overlap). Possible adenylate cyclase (EC 4.6.1.1) (see second citation below). Some similarity at the C-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores: opt: 277, E(): 2e-12, (34.0% identity in 156 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1318c|MTCY130.03c (541 aa), FASTA scores: opt: 2423, E(): 0, (68.7% identity in 534 aa overlap); Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 2354, E(): 0, (66.3% identity in 534 aa overlap). N-terminus is hydrophobic. BELONGS TO ADENYLYL CYCLASE CLASS-3 FAMILY. Protein product from Mb1354c detected using SWATH mass spectrometry. Mb1354c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P59972" /db_xref="InterPro:IPR001054" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR029787" /db_xref="UniProtKB/Swiss-Prot:P59972" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MPSEKATTRH LPGAVETLSP RTGRRPETPA YGSWLLGRVS ESPRMRRVRI QGMLTVAILV 61 TNVIGLIVGA MLLTVAFPKP SVILDAPHWV SFGIVPGYCV LAFILGTYWL TRQTARALRW 121 AIEERTPSHD EARSAFLVPL RVALAVLFLW GAAAALWTII YGLANRLFIP RFLFSMGVIG 181 VVAATSCYLL TEFALRPMAA QALEVGATPR SLVRGIVGRT MLVWLLCSGV PNVGVALTAI 241 FDDTFWELSN DQFMITVLIL WAPLLIFGFI LMWILAWLTA TPVRVVREAL NRVEQGDLSG 301 DLVVFDGTEL GELQRGFNRM VEGLRERERV RDLFGRHVGR EVAAAAERER PKLGGEERHV 361 AVVFVDIVGS TQLVTSRPAA EVVMLLNRFF TVIVDEVNHH RGLVNKFQGD ASLAVFGAPN 421 RLSHPEDAAL ATARAIADRL ASEMPECQAG IGVAAGQVVA GNVGAHERFE YTVIGEPVNE 481 AARLCELAKS YPSRLLASSQ TLRGASENEC ARWSLGETVT LRGHDQPIRL ASPVQQLQMP 541 AQSADIVGGA LGDHQTHTIY RGAHPTD //