LOCUS SIT99956.1 535 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE ADENYLATE CYCLASE (ATP PYROPHOSPHATE-LYASE) (ADENYLYL CYCLASE) protein. ACCESSION LT708304-1368 PROTEIN_ID SIT99956.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1353C" /note="Mb1353c, -, len: 535 aa. Equivalent to Rv1319c, len: 535 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 535 aa overlap). Possible adenylate cyclase (EC 4.6.1.1). Some similarity at the C-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores: opt: 254, E(): 2.4e-10, (33.3% identity in 144 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1318c|MTCY130.03c (541 aa), FASTA scores: opt: 2505, E(): 0, (71.0% identity in 534 aa overlap); Rv1320c|MTCY130.05c (567 aa), FASTA scores: opt: 2354, E(): 0, (66.3% identity in 534 aa overlap). N-terminus is hydrophobic. BELONGS TO ADENYLYL CYCLASE CLASS-3 FAMILY. Protein product from Mb1353c detected using SWATH mass spectrometry. Mb1353c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A4Y3" /db_xref="InterPro:IPR001054" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR029787" /db_xref="UniProtKB/Swiss-Prot:P0A4Y3" BEGIN 1 MPAKKTMAQR LGQALETMTR QCGQLPETPA YGSWLLGRVS ESPSRRWVRI KRIVTVYIMT 61 ANLTGIVVAL LVVTFAFPVP SIYTDAPWWV TFGVAPAYAT LALAIGTYWI TTRIVRASIR 121 WAIEERAPSQ ADGRNTLLLP FRVAAVHLIL WDIGGALLAT LYGLANRVFV TIILFSVTIC 181 GVLVATNCYL FTEFALRPVA AKALEAGRPP RRFAPGIMGR TMTVWSLGSG VPVTGIATTA 241 LYVLLVHNLT ETQLASAVLI LSITTLIFGF LVMWILAWLT AAPVRVVRAA LKRVEQGDLR 301 GDLVVFDGTE LGELQRGFNA MVNGLRERER VRDLFGRHVG REVAAAAERE RPQLGGEDRH 361 AAVVFVDIVG STQLVDNQPA AHVVKLLNRF FAIVVNEVDR HHGLINKFAG DAALAIFGAP 421 NRLDRPEDAA LAAARAIADR LANEMPEVQA GIGVAAGQIV AGNVGAKQRF EYTVVGKPVN 481 QAARLCELAK SHPARLLASS DTLHAASETE RAHWSLGETV TLRGHEQPTR LAVPT //