LOCUS SIT99953.1 416 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PUTATIVE ADA REGULATORY PROTEIN ALKAb [SECOND PART] (Regulatory protein of adaptative response) : Methylated-DNA--protein- cysteine methyltransferase (O-6-methylguanine-DNA alkyltransferase) protein. ACCESSION LT708304-1365 PROTEIN_ID SIT99953.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="alkAb" /locus_tag="BQ2027_MB1350C" /note="Mb1350c, alkAb, len: 416 aa. Equivalent to the 3' end of Rv1317c, len: 496 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 416 aa overlap). Putative alkA (alternate gene name: ada), regulatory protein (EC 2.1.1.63), similar to 3MG2_ECOLI|P04395 dna-3-methyladenine glycosidase II from Escherichia coli (282 aa), FASTA scores, opt: 437, E(): 8.6e-22, (32.8% identity in 293 aa overlap), also similar to other ada proteins e.g. ADA_SALTY|P26189 Salmonella typhimurium (352 aa), FASTA scores: E(): 5.3e-08, (35.9% identity in 156 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, alkA exists as a single gene. In Mycobacterium bovis, a single base transition (g-a) introduces a premature stop codon that splits alkA into 2 parts, alkAa and alkAb. Mb1350c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y043" /db_xref="InterPro:IPR003265" /db_xref="InterPro:IPR009057" /db_xref="InterPro:IPR010316" /db_xref="InterPro:IPR011257" /db_xref="InterPro:IPR018060" /db_xref="InterPro:IPR018062" /db_xref="InterPro:IPR023170" /db_xref="InterPro:IPR037046" /db_xref="UniProtKB/TrEMBL:A0A1R3Y043" BEGIN 1 MRSDVVARAM RLIADGTVDR DGVSGLAAQL GYTIRQLERL LQAVVGAGPL ALARAQRMQT 61 ARVLIETTNL PFGDVAFAAG FSSIRQFNDT VRLACDGTPT ALRARAAARF ESATASAGTV 121 SLRLPVRAPF AFEGVFGHLA ATAVPGCEEV RDGAYRRTLR LPWGNGIVSL TPAPDHVRCL 181 LVLDDFRDLM TATARCRRLL DLDADPEAIV EALGADPDLR AVVGKAPGQR IPRTVDEAEF 241 AVRAVLAQQV STKAASTHAG RLVAAYGRPV HDRHGALTHT FPSIEQLAEI DPGHLAVPKA 301 RQRTINALVA SLADKSLVLD AGCDWQRARG QLLALPGVGP WTAEVIAMRG LGDPDAFPAS 361 DLGLRLAAKK LGLPAQRRAL TVHSARWRPW RSYATQHLWT TLEHPVNQWP PQEKIA //