LOCUS SIT99947.1 144 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CONSERVED HYPOTHETICAL SECRETED PROTEIN protein. ACCESSION LT708304-1359 PROTEIN_ID SIT99947.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1344" /note="Mb1344, -, len: 144 aa. Equivalent to Rv1312, len: 147 aa, from Mycobacterium tuberculosis strain H37Rv, (99.3% identity in 142 aa overlap). Conserved hypothetical secreted protein with potential N-terminal signal sequence. Highly similar to P53432|Y02W_MYCLE hypothetical Mycobacterium leprae protein (147 aa), FASTA score: opt: 884, E(): 0, (88.4% identity in 147 aa overlap). N-terminus hydrophobic. REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a 2 bp deletion (cg-*) at the 3' end leads to a slightly shorter product compared to Mycobacterium tuberculosis strain H37Rv (144 aa versus 147 aa). Protein product from Mb1344 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1344 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XXZ8" /db_xref="InterPro:IPR019675" /db_xref="UniProtKB/TrEMBL:A0A1R3XXZ8" BEGIN 1 MSVPMIGMVV LVVVLGLAVL ALSYRLWKLR QGGTAGIMRD IPAVGGHGWR HGVIRYRGGE 61 AAFYRLSSLR LWPDRRLSRR GVEIISRRAP RGDEFDIMTD EIVVVELCDS TQDRRVGYEI 121 ALDRGALTAF LSWLESRPSP RAPP //