LOCUS SIT99935.1 325 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE HEMK PROTEIN HOMOLOG HEMK protein. ACCESSION LT708304-1347 PROTEIN_ID SIT99935.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="hemK" /locus_tag="BQ2027_MB1332" /note="Mb1332, hemK, len: 325 aa. Equivalent to Rv1300, len: 325 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 325 aa overlap). Probable hemK protein homolog (EC 2.1.1.-), homology suggests translation may start at aa 22, highly similar to many e.g. HEMK_MYCLE|P45832 Mycobacterium leprae (288 aa), FASTA scores: opt: 936, E(): 0, (76.7% identity in 189 aa overlap). BELONGS TO THE HEMK FAMILY OF MODIFICATION METHYLASES. Mb1332 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XYA7" /db_xref="InterPro:IPR002052" /db_xref="InterPro:IPR004556" /db_xref="InterPro:IPR019874" /db_xref="InterPro:IPR029063" /db_xref="InterPro:IPR040758" /db_xref="InterPro:IPR041698" /db_xref="UniProtKB/TrEMBL:A0A1R3XYA7" BEGIN 1 MTSAPATMRW GNLPLAGESG TMTLRQAIDL AAALLAEAGV DSARCDAEQL AAHLAGTDRG 61 RLPLFEPPGD EFFGRYRDIV TARARRVPLQ HLIGTVSFGP VVLHVGPGVF VPRPETEAIL 121 AWATAQSLPA RPLIVDACTG SGALAVALAQ HRANLGLKAR IIGIDDSDCA LDYARRNAAG 181 TPVELVRADV TTPCLLPELD GQVDLMVSNP PYIPDAAVLE PEVAQHDPHH ALFGGPDGMT 241 VISAVVGLAG RWLRPGGLFA VEHDDTTSSS TVDLVSSTKL FVDVQARKDL AGRPRFVTAM 301 RWGHLPLAGE NGAIDPRQRR CRAKR //