LOCUS       SIT99920.1               614 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            BIFUNCTIONAL ENZYME CYSN/CYSC: SULFATE ADENYLTRANSFERASE
            (SUBUNIT 1) + ADENYLYLSULFATE KINASE protein.
ACCESSION   LT708304-1332
PROTEIN_ID  SIT99920.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cysN"
                     /locus_tag="BQ2027_MB1317"
                     /note="Mb1317, cysN, len: 614 aa. Equivalent to Rv1286,
                     len: 614 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 614 aa overlap). Probable cysN/cysC
                     bifunctional enzyme, sulfate adenylyltransferase subunit 1
                     (EC 2.7.7.4) and Adenylylsulfate kinase (EC 2.7.1.25) (see
                     first citation below), similar to CYSN_ECOLI|P23845
                     sulfate adenylate transferase subunit 1 from Escherichia
                     coli (475 aa), FASTA scores: opt: 1291, E():0, (50.2%
                     identity in 428 aa overlap). Contains 2 x PS00017
                     ATP/GTP-binding site motif A, PS00301 GTP-binding
                     elongation factors signature. Protein product from Mb1317
                     detected using shotgun mass spectrometry. Mb1317 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3XXW4"
                     /db_xref="InterPro:IPR000795"
                     /db_xref="InterPro:IPR002891"
                     /db_xref="InterPro:IPR009000"
                     /db_xref="InterPro:IPR009001"
                     /db_xref="InterPro:IPR011779"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="InterPro:IPR031157"
                     /db_xref="InterPro:IPR041757"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XXW4"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MTTLLRLATA GSVDDGKSTL IGRLLYDSKA VMEDQWASVE QTSKDRGHDY TDLALVTDGL
       61 RAEREQGITI DVAYRYFATP KRKFIIADTP GHIQYTRNMV TGASTAQLVI VLVDARHGLL
      121 EQSRRHAFLA SLLGIRHLVL AVNKMDLLGW DQEKFDAIRD EFHAFAARLD VQDVTSIPIS
      181 ALHGDNVVTK SDQTPWYEGP SLLSHLEDVY IAGDRNMVDV RFPVQYVIRP HTLEHQDHRS
      241 YAGTLASGVM RSGDEVVVLP IGKTTRITAI DGPNGPVAEA FPPMAVSVRL ADDIDISRGD
      301 MIARTHNQPR ITQEFDATVC WMADNAVLEP GRDYVVKHTT RTVRARIAGL DYRLDVNTLH
      361 RDKTATALKL NELGRVSLRT QVPLLLDEYT RNASTGSFIL IDPDTNGTVA AGMVLRDVSA
      421 RTPSPNTVRH RSLVTAQDRP PRGKTVWFTG LSGSGKSSVA MLVERKLLEK GISAYVLDGD
      481 NLRHGLNADL GFSMADRAEN LRRLSHVATL LADCGHLVLV PAISPLAEHR ALARKVHADA
      541 GIDFFEVFCD TPLQDCERRD PKGLYAKARA GEITHFTGID SPYQRPKNPD LRLTPDRSID
      601 EQAQEVIDLL ESSS
//