LOCUS SIT99920.1 614 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE BIFUNCTIONAL ENZYME CYSN/CYSC: SULFATE ADENYLTRANSFERASE (SUBUNIT 1) + ADENYLYLSULFATE KINASE protein. ACCESSION LT708304-1332 PROTEIN_ID SIT99920.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cysN" /locus_tag="BQ2027_MB1317" /note="Mb1317, cysN, len: 614 aa. Equivalent to Rv1286, len: 614 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 614 aa overlap). Probable cysN/cysC bifunctional enzyme, sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) and Adenylylsulfate kinase (EC 2.7.1.25) (see first citation below), similar to CYSN_ECOLI|P23845 sulfate adenylate transferase subunit 1 from Escherichia coli (475 aa), FASTA scores: opt: 1291, E():0, (50.2% identity in 428 aa overlap). Contains 2 x PS00017 ATP/GTP-binding site motif A, PS00301 GTP-binding elongation factors signature. Protein product from Mb1317 detected using shotgun mass spectrometry. Mb1317 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXW4" /db_xref="InterPro:IPR000795" /db_xref="InterPro:IPR002891" /db_xref="InterPro:IPR009000" /db_xref="InterPro:IPR009001" /db_xref="InterPro:IPR011779" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR031157" /db_xref="InterPro:IPR041757" /db_xref="UniProtKB/TrEMBL:A0A1R3XXW4" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTTLLRLATA GSVDDGKSTL IGRLLYDSKA VMEDQWASVE QTSKDRGHDY TDLALVTDGL 61 RAEREQGITI DVAYRYFATP KRKFIIADTP GHIQYTRNMV TGASTAQLVI VLVDARHGLL 121 EQSRRHAFLA SLLGIRHLVL AVNKMDLLGW DQEKFDAIRD EFHAFAARLD VQDVTSIPIS 181 ALHGDNVVTK SDQTPWYEGP SLLSHLEDVY IAGDRNMVDV RFPVQYVIRP HTLEHQDHRS 241 YAGTLASGVM RSGDEVVVLP IGKTTRITAI DGPNGPVAEA FPPMAVSVRL ADDIDISRGD 301 MIARTHNQPR ITQEFDATVC WMADNAVLEP GRDYVVKHTT RTVRARIAGL DYRLDVNTLH 361 RDKTATALKL NELGRVSLRT QVPLLLDEYT RNASTGSFIL IDPDTNGTVA AGMVLRDVSA 421 RTPSPNTVRH RSLVTAQDRP PRGKTVWFTG LSGSGKSSVA MLVERKLLEK GISAYVLDGD 481 NLRHGLNADL GFSMADRAEN LRRLSHVATL LADCGHLVLV PAISPLAEHR ALARKVHADA 541 GIDFFEVFCD TPLQDCERRD PKGLYAKARA GEITHFTGID SPYQRPKNPD LRLTPDRSID 601 EQAQEVIDLL ESSS //