LOCUS       SIT99919.1               332 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            SULFATE ADENYLYLTRANSFERASE SUBUNIT 2 CYSD protein.
ACCESSION   LT708304-1331
PROTEIN_ID  SIT99919.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cysD"
                     /locus_tag="BQ2027_MB1316"
                     /note="Mb1316, cysD, len: 332 aa. Equivalent to Rv1285,
                     len: 332 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 332 aa overlap). Probable cysD, sulfate
                     adenylyltransferase subunit 2 (EC 2.7.7.4) (see first
                     citation below), homology suggests start site at aa 24 or
                     28, similar to e.g. CYSD_ECOLI|P21156 sulfate adenylate
                     transferase subunit 2 from Escherichia coli (302 aa),
                     FASTA score: opt: 973, E():0, (52.5% identity in 303 aa
                     overlap). Also similar to Mycobacterium tuberculosis
                     Rv2392, 3'-phosphoadenylylsulfate reductase. BELONGS TO
                     THE PAPS REDUCTASE FAMILY. CYSD SUBFAMILY. Thought to be
                     differentially expressed within host cells (see third
                     citation below). Protein product from Mb1316 detected
                     using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb1316 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P65671"
                     /db_xref="InterPro:IPR002500"
                     /db_xref="InterPro:IPR011784"
                     /db_xref="InterPro:IPR014729"
                     /db_xref="UniProtKB/Swiss-Prot:P65671"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MAITINMVNP TGFIRYEDVE QEAMTSDVTV GPAPGQYQLS HLRLLEAEAI HVIREVAAEF
       61 ERPVLLFSGG KDSIVMLHLA LKAFRPGRLP FPVMHVDTGH NFDEVIATRD ELVAAAGVRL
      121 VVASVQDDID AGRVVETIPS RNPIQTVTLL RAIRENQFDA AFGGARRDEE KARAKERVFS
      181 FRDEFGQWDP KAQRPELWNL YNGRHHKGEH IRVFPLSNWT EFDIWSYIGA EQVRLPSIYF
      241 AHRRKVFQRD GMLLAVHRHM QPRADEPVFE ATVRFRTVGD VTCTGCVESS ASTVAEVIAE
      301 TAVARLTERG ATRADDRISE AGMEDRKRQG YF
//