LOCUS SIT99886.1 563 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE COLD-SHOCK DEAD-BOX PROTEIN A HOMOLOG DEAD (ATP-dependent RNA helicase deaD homolog) protein. ACCESSION LT708304-1298 PROTEIN_ID SIT99886.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="deaD" /locus_tag="BQ2027_MB1285" /note="Mb1285, deaD, len: 563 aa. Equivalent to Rv1253, len: 563 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 563 aa overlap). Probable Dead, Cold-shock DEAD-box protein A homolog, similar to many e.g. DEAD_ECOLI|P23304 Escherichia coli (646 aa), FASTA scores: opt: 1490, E(): 0, (46.7% identity in 578 aa overlap); similar to Mycobacterium tuberculosis Rv3211. Contains PS00017 ATP/GTP-binding site motif A, PS00039 DEAD-box subfamily ATP-dependent helicases signature. BELONGS TO THE DEAD BOX FAMILY HELICASE. Protein product from Mb1285 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1285 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXT6" /db_xref="InterPro:IPR000629" /db_xref="InterPro:IPR001650" /db_xref="InterPro:IPR005580" /db_xref="InterPro:IPR011545" /db_xref="InterPro:IPR012677" /db_xref="InterPro:IPR014001" /db_xref="InterPro:IPR014014" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR028618" /db_xref="InterPro:IPR034415" /db_xref="UniProtKB/TrEMBL:A0A1R3XXT6" BEGIN 1 MAFPEYSPAA SAATFADLQI HPRVLRAIGD VGYESPTAIQ AATIPALMAG SDVVGLAQTG 61 TGKTAAFAIP MLSKIDITSK VPQALVLVPT RELALQVAEA FGRYGAYLSQ LNVLPIYGGS 121 SYAVQLAGLR RGAQVVVGTP GRVIDHLERA TLDLSRVDFL VLDEADEMLT MGFADDVERI 181 LSETPEYKQV ALFSATMPPA IRKLSAKYLH DPFEVTCKAK TAVAENISQS YIQVARKMDA 241 LTRVLEVEPF EAMIVFVRTK QATEEIAEKL RARGFSAAAI SGDVPQAQRE RTITALRDGD 301 IDILVATDVA ARGLDVERIS HVLNYDIPHD TESYVHRIGR TGRAGRSGAA LIFVSPRELH 361 LLKAIEKATR QTLTEAQLPT VEDVNTQRVA KFADSITNAL GGPGIELFRR LVEEYEREHD 421 VPMADIAAAL AVQCRGGEAF LMAPDPPLSR RNRDQRRDRP QRPKRRPDLT TYRVAVGKRH 481 KIGPGAIVGA IANEGGLHRS DFGQIRIGPD FSLVELPAKL PRATLKKLAQ TRISGVLIDL 541 RPYRPPDAAR RHNGGKPRRK HVG //