LOCUS SIT99878.1 276 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE protein. ACCESSION LT708304-1290 PROTEIN_ID SIT99878.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1277C" /note="Mb1277c, -, len: 276 aa. Equivalent to Rv1245c, len: 276 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 276 aa overlap). Probable short-chain dehydrogenase/reductase (EC 1.-.-.-), equivalent to NP_301801.1|NC_002677 short chain alcohol dehydrogenase from Mycobacterium leprae (277 aa). Also highly similar to various dehydrogenases and oxidoreductases e.g. NP_250228.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (295 aa); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv3085|MTV013.06 PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE (276 aa), FASTA scores: opt: 368, E(): 1.2e-16, (35.3% identity in 224 aa overlap); Rv3057c|MTCY22D7.24 PUTATIVE SHORT CHAIN ALCOHOL DEHYDROGENASE/REDUCTASE (287 aa), FASTA scores: opt: 471, E(): 1.3e-21, (32.4% identity in 281 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. BELONGS TO THE SHORT-CHAIN DEHYDROGENASES/REDUCTASES (SDR) FAMILY. Protein product from Mb1277c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1277c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXS4" /db_xref="InterPro:IPR002347" /db_xref="InterPro:IPR020904" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3XXS4" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MEGFAGKVAV VTGAGSGIGQ ALAIELARSG AKVAISDVDT DGLADTEHRL KAISTPVKTD 61 RLDVTEREAF LAYADAVNEH FGTVNQIYNN AGIAFTGDIE VSQFKDIERV MDVDFWGVVN 121 GTKAFLPHLI ASGDGHVINI SSVFGLFSAP GQAAYNSAKF AVRGFTEALR QEMALAGHPV 181 KVTTVHPGGV KTAIARNATA AEGLDQAELA ETFDKRVAHL SPQRAAQIIL TGVAKNKARV 241 LVGVDAKVLD LVVRLTGSGY QRIFPIITGR LIPRPR //