LOCUS SIT99856.1 528 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SERINE PROTEASE HTRA (DEGP PROTEIN) protein. ACCESSION LT708304-1268 PROTEIN_ID SIT99856.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="htrA" /locus_tag="BQ2027_MB1255" /note="Mb1255, htrA, len: 528 aa. Equivalent to Rv1223, len: 528 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 528 aa overlap). Probable htrA (alternate gene name: degP), serine protease precursor (EC 3.4.21.-), equivalent to U15180|MLU15180_31|Q49972|ML1078|HTRA POSSIBLE SERINE PROTEASE from Mycobacterium leprae (533 aa), FASTA scores: opt: 2777, E(): 4.1e-141, (81.6% identity in 533 aa overlap). Also similar to many others e.g. HTRA_ECOLI|P09376 protease do precursor from Escherichia coli (EC 3.4.21.-) (474 aa), FASTA scores: opt: 581, E(): 9.1e-27, (36.3% identity in 278 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Start changed since first submission (-21 aa). Protein product from Mb1255 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1255 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXQ7" /db_xref="InterPro:IPR001478" /db_xref="InterPro:IPR001940" /db_xref="InterPro:IPR009003" /db_xref="InterPro:IPR036034" /db_xref="UniProtKB/TrEMBL:A0A1R3XXQ7" BEGIN 1 MDTRVDTDNA MPARFSAQIQ NEDEVTSDQG NNGGPNGGGR LAPRPVFRPP VDPASRQAFG 61 RPSGVQGSFV AERVRPQKYQ DQSDFTPNDQ LADPVLQEAF GRPFAGAESL QRHPIDAGAL 121 AAEKDGAGPD EPDDPWRDPA AAAALGTPAL AAPAPHGALA GSGKLGVRDV LFGGKVSYLA 181 LGILVAIALV IGGIGGVIGR KTAEVVDAFT TSKVTLSTTG NAQEPAGRFT KVAAAVADSV 241 VTIESVSDQE GMQGSGVIVD GRGYIVTNNH VISEAANNPS QFKTTVVLND GKEVPANLVG 301 RDPKTDLAVL KVDNVDNLTV ARLGDSSKVR VGDEVLAVGA PLGLRSTVTQ GIVSALHRPV 361 PLSGEGSDTD TVIDAIQTDA SINHGNSGGP LIDMDAQVIG INTAGKSLSD SASGLGFAIP 421 VNEMKLVANS LIKDGKIVHP TLGINTRSVS NAIASGAQVA NVKAGSPAQK GGILENDVIV 481 KVGNRAVADS DEFVVAVRQL AIGQDAPIEV VREGRHVTLT VKPDPDST //