LOCUS       SIT99829.1               390 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 ppe
            family protein ppe18 protein.
ACCESSION   LT708304-1241
PROTEIN_ID  SIT99829.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="PPE18"
                     /locus_tag="BQ2027_MB1228"
                     /standard_name="mtb39a"
                     /note="Mb1228, PPE18, len: 390 aa. Equivalent to Rv1196,
                     len: 391 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.0% identity in 391 aa overlap). PPE18 (alternate gene
                     name: mtb39a). Member of the Mycobacterium tuberculosis
                     PPE family of glycine-rich proteins, highly similar to
                     others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa
                     protein cy02b10.25c (396 aa), FASTA scores: opt: 2124,
                     E(): 0, (85.1% identity in 397 aa overlap). Note that
                     expression of Rv1196 was demonstrated in lysates by
                     immunodetection (see first citation below).
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a
                     14 bp to 11 bp substitution leads to a slightly shorter
                     product compared to its homolog in Mycobacterium
                     tuberculosis strain H37Rv (390 aa versus 391 aa). Protein
                     product from Mb1228 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb1228 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="InterPro:IPR000030"
                     /db_xref="InterPro:IPR022171"
                     /db_xref="InterPro:IPR038332"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XXN5"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MVDFGALPPE INSARMYAGP GSASLVAAAQ MWDSVASDLF SAASAFQSVV WGLTVGSWIG
       61 SSAGLMVAAA SPYVAWMSVT AGQAELTAAQ VRVAAAAYET AYGLTVPPPV IAENRAELMI
      121 LIATNLLGQN TPAIAVNEAE YGEMWAQDAA AMFGYAAATA TATATLLPFE EAPEMTSAGG
      181 LLEQAAAVEE ASDTAAANQL MNNVPQALQQ LAQPTQGTTP SSKLGGLWKT VSPHLSPISN
      241 MVSMANNHVS MTNSGVSMTN TLSSMLKGFA PAAAQAVETA AQNGVRAMSS LGSSLGSSGL
      301 GGGVAANLGR AASVGSLSVP QAWAAANQAV TPAARALPLT SLTSAAERGP GQMLGGLPVG
      361 QMGARAGGGL SGVLRVPPRP YVMPHSPAAG
//