LOCUS SIT99829.1 390 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ppe family protein ppe18 protein. ACCESSION LT708304-1241 PROTEIN_ID SIT99829.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="PPE18" /locus_tag="BQ2027_MB1228" /standard_name="mtb39a" /note="Mb1228, PPE18, len: 390 aa. Equivalent to Rv1196, len: 391 aa, from Mycobacterium tuberculosis strain H37Rv, (99.0% identity in 391 aa overlap). PPE18 (alternate gene name: mtb39a). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa protein cy02b10.25c (396 aa), FASTA scores: opt: 2124, E(): 0, (85.1% identity in 397 aa overlap). Note that expression of Rv1196 was demonstrated in lysates by immunodetection (see first citation below). REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a 14 bp to 11 bp substitution leads to a slightly shorter product compared to its homolog in Mycobacterium tuberculosis strain H37Rv (390 aa versus 391 aa). Protein product from Mb1228 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1228 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR000030" /db_xref="InterPro:IPR022171" /db_xref="InterPro:IPR038332" /db_xref="UniProtKB/TrEMBL:A0A1R3XXN5" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MVDFGALPPE INSARMYAGP GSASLVAAAQ MWDSVASDLF SAASAFQSVV WGLTVGSWIG 61 SSAGLMVAAA SPYVAWMSVT AGQAELTAAQ VRVAAAAYET AYGLTVPPPV IAENRAELMI 121 LIATNLLGQN TPAIAVNEAE YGEMWAQDAA AMFGYAAATA TATATLLPFE EAPEMTSAGG 181 LLEQAAAVEE ASDTAAANQL MNNVPQALQQ LAQPTQGTTP SSKLGGLWKT VSPHLSPISN 241 MVSMANNHVS MTNSGVSMTN TLSSMLKGFA PAAAQAVETA AQNGVRAMSS LGSSLGSSGL 301 GGGVAANLGR AASVGSLSVP QAWAAANQAV TPAARALPLT SLTSAAERGP GQMLGGLPVG 361 QMGARAGGGL SGVLRVPPRP YVMPHSPAAG //