LOCUS SIT99824.1 304 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Putative hydrolase protein. ACCESSION LT708304-1236 PROTEIN_ID SIT99824.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1223" /note="Mb1223, -, len: 304 aa. Equivalent to Rv1191, len: 304 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 304 aa overlap). Conserved hypothetical protein, similar to Q54528 RDMC from Streptomyces purpurascens (298 aa), FASTA scores: opt: 196, E(): 1.5e-05, (27.5% identity in 269 aa overlap); Rv0134|MTCI5.08 (300 aa), FASTA scores: opt: 197, E(): 6.6e-06, (26.4% identity in 299 aa overlap), some similarity to PIP_NEIGO|P42786 proline iminopeptidase (EC 3.4.11.5) (310 aa), FASTA scores: opt: 196, E(): 1.3e-05, (32.2% identity in 152 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature. Protein product from Mb1223 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1223 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR000073" /db_xref="InterPro:IPR029058" /db_xref="UniProtKB/TrEMBL:A0A1R3XXN6" BEGIN 1 MAVAIARPKL EGNIAVGEDR RIGFAEFGAP QGRAVFWLHG TPGARRQIPT EARVYAEHHN 61 IRLIGVDRPG IGASTPHQYE TILAFADDLR TIADTLGIDK MAVVGLSGGG PYTLACAAGL 121 PDRVVAAGVL GGVAPTRGPD AISGGLMRLG SAVAPLLQVG GTPLRLGASL LIRAARPVAS 181 PALDLYGLLS PRADRHLLAR PEFKAMFLDD LLNGSRKQLA APFADVIAFA RDWGFRLDEV 241 KVPVRWWHGD HDHIVPFSHG EHVVSRLPDA KLLHLPGESH LAGLGRGEEI LSTLMQIWDR 301 DLRK //