LOCUS SIT99822.1 290 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE ALTERNATIVE RNA POLYMERASE SIGMA FACTOR SIGI protein. ACCESSION LT708304-1234 PROTEIN_ID SIT99822.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="sigI" /locus_tag="BQ2027_MB1221" /note="Mb1221, sigI, len: 290 aa. Equivalent to Rv1189, len: 290 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 290 aa overlap). Possible sigI, alternative RNA polymerase sigma factor (see citation below), similar to several e.g. O05767|U87307 extracytoplasmic function alternative sigma factor (sigE) from M. smegmatis (204 aa), FASTA scores: opt: 239, E(): 1.3e-09, (32.9% identity in 167 aa overlap). Mb1221 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XZQ9" /db_xref="InterPro:IPR007627" /db_xref="InterPro:IPR013249" /db_xref="InterPro:IPR013324" /db_xref="InterPro:IPR013325" /db_xref="InterPro:IPR014284" /db_xref="InterPro:IPR032710" /db_xref="InterPro:IPR036388" /db_xref="UniProtKB/TrEMBL:A0A1R3XZQ9" BEGIN 1 MSQHDPVSAA WRAHRAYLVD LAFRMVGDIG VAEDMVQEAF SRLLRAPVGD IDDERGWLIV 61 VTSRLCLDHI KSASTRRERP QDIAAWHDGD ASVSSVDPAD RVTLDDEVRL ALLIMLERLG 121 PAERVVFVLH EIFGLPYQQI ATTIGSQAST CRQLAHRARR KINESRIAAS VEPAQHRVVT 181 RAFIEACSNG DLDTLLEVLD PGVAGEIDAR KGVVVVGADR VGPTILRHWS HPATVLVAQP 241 VCGQPAVLAF VNRALAGVLA LSIEAGKITK IHVLVQPSTL DPLRAELGGG //