LOCUS SIT99821.1 329 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PROLINE DEHYDROGENASE protein. ACCESSION LT708304-1233 PROTEIN_ID SIT99821.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1220" /note="Mb1220, -, len: 329 aa. Equivalent to Rv1188, len: 329 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 329 aa overlap). Possible putA, proline dehydrogenase (EC 1.5.99.8), similar to part of Q52711|X78346 proline dehydrogenase from Rhodobacter capsulatus (1127 aa), FASTA scores: opt: 194, E(): 1.5e-07, (31.2% identity in 349 aa overlap). Also similar to two Bacillus subtilis proline dehydrohenases E1184363|Z99120 (302 aa), FASTA scores: opt: 509, E(): 0, (37.1% identity in 313 aa overlap); and E1182272|Z99105 (303 aa), FASTA scores: opt: 513, E(): 0, (32.5% identity in 311 aa overlap). Highly similar to AL035569|SC8D9.31 Streptomyces coelicolor (308 aa), FASTA scores: opt: 984, E(): 0, (50.0% identity in 312 aa overlap). Protein product from Mb1220 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1220 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXM0" /db_xref="InterPro:IPR002872" /db_xref="InterPro:IPR008219" /db_xref="InterPro:IPR015659" /db_xref="InterPro:IPR029041" /db_xref="UniProtKB/TrEMBL:A0A1R3XXM0" BEGIN 1 MAGWFAHTLR PAMLAAGRSD RLGRIVERSP LTRGVVRRFV PGDTLDDVVD IVTALRDSGR 61 YLSIDYLGEN VTDADDAAAA VRAYLGLLDV LGRRGDIACD GVRPLEVSLK LSALGQALDR 121 DGQKIALDNA RAICERAERV GAWVTVDAED HTTTDSTLSI SGDLRVDFPW LGTVVQAYLR 181 RTLADCAELA AVGARVRLCK GAYDEPASVA YRDAAQVTDS YLRCLRVLTA GRGYPMVATH 241 DPVIIAAVPG ITRESGRSQG DFEYQMLYGV RDDEQRRLTG AGNHVRVYVP FGTRWYGYFL 301 RRLAERPANL AFFLRALTDR RRARGCAER //