LOCUS SIT99820.1 543 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PYRROLINE-5-CARBOXYLATE DEHYDROGENASE ROCA protein. ACCESSION LT708304-1232 PROTEIN_ID SIT99820.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="rocA" /locus_tag="BQ2027_MB1219" /note="Mb1219, rocA, len: 543 aa. Equivalent to Rv1187, len: 543 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 543 aa overlap). Probable rocA, pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12), similar to many e.g. PUT2_HUMAN|P30038 human delta-1-pyrroline-5-carboxylate dehydrogenase (563 aa), FASTA scores: opt: 1596, E():0, (46.0% identity in 531 aa overlap). Also similar to other Mycobacterium tuberculosis hypothetical dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site. Protein product from Mb1219 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1219 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXL2" /db_xref="InterPro:IPR005931" /db_xref="InterPro:IPR015590" /db_xref="InterPro:IPR016160" /db_xref="InterPro:IPR016161" /db_xref="InterPro:IPR016162" /db_xref="InterPro:IPR016163" /db_xref="UniProtKB/TrEMBL:A0A1R3XXL2" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MDAITQVPVP ANEPVHDYAP KSPERTRLRT ELASLADHPI DLPHVIGGRH RMGDGERIDV 61 VQPHRHAARL GTLTNATHAD AAAAVEAAMS AKSDWAALPF DERAAVFLRA ADLLAGPWRE 121 KIAAATMLGQ SKSVYQAEID AVCELIDFWR FNVAFARQIL EQQPISGPGE WNRIDYRPLD 181 GFVYAITPFN FTSIAGNLPT APALMGNTVI WKPSITQTLA AYLTMQLLEA AGLPPGVINL 241 VTGDGFAVSD VALADPRLAG IHFTGSTATF GHLWQWVGTN IGRYHSYPRL VGETGGKDFV 301 VAHASARPDV LRTALIRGAF DYQGQKCSAV SRAFIAHSVW QRMGDELLAK AAELRYGDIT 361 DLSNYGGALI DQRAFVKNVD AIERAKGAAA VTVAVGGEYD DSEGYFVRPT VLLSDDPTDE 421 SFVIEYFGPL LSVHVYPDER YEQILDVIDT GSRYALTGAV IADDRQAVLT ALDRLRFAAG 481 NFYVNDKPTG AVVGRQPFGG ARGSGTNDKA GSPLNLLRWT SARSIKETFV AATDHIYPHM 541 AVD //