LOCUS       SIT99820.1               543 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            PYRROLINE-5-CARBOXYLATE DEHYDROGENASE ROCA protein.
ACCESSION   LT708304-1232
PROTEIN_ID  SIT99820.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="rocA"
                     /locus_tag="BQ2027_MB1219"
                     /note="Mb1219, rocA, len: 543 aa. Equivalent to Rv1187,
                     len: 543 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 543 aa overlap). Probable rocA,
                     pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12),
                     similar to many e.g. PUT2_HUMAN|P30038 human
                     delta-1-pyrroline-5-carboxylate dehydrogenase (563 aa),
                     FASTA scores: opt: 1596, E():0, (46.0% identity in 531 aa
                     overlap). Also similar to other Mycobacterium tuberculosis
                     hypothetical dehydrogenases e.g. Rv0768, Rv2858c, etc.
                     Contains PS00687 Aldehyde dehydrogenases glutamic acid
                     active site and PS00070 Aldehyde dehydrogenases cysteine
                     active site. Protein product from Mb1219 detected using
                     shotgun mass spectrometry and SWATH mass spectrometry.
                     Mb1219 found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3XXL2"
                     /db_xref="InterPro:IPR005931"
                     /db_xref="InterPro:IPR015590"
                     /db_xref="InterPro:IPR016160"
                     /db_xref="InterPro:IPR016161"
                     /db_xref="InterPro:IPR016162"
                     /db_xref="InterPro:IPR016163"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XXL2"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MDAITQVPVP ANEPVHDYAP KSPERTRLRT ELASLADHPI DLPHVIGGRH RMGDGERIDV
       61 VQPHRHAARL GTLTNATHAD AAAAVEAAMS AKSDWAALPF DERAAVFLRA ADLLAGPWRE
      121 KIAAATMLGQ SKSVYQAEID AVCELIDFWR FNVAFARQIL EQQPISGPGE WNRIDYRPLD
      181 GFVYAITPFN FTSIAGNLPT APALMGNTVI WKPSITQTLA AYLTMQLLEA AGLPPGVINL
      241 VTGDGFAVSD VALADPRLAG IHFTGSTATF GHLWQWVGTN IGRYHSYPRL VGETGGKDFV
      301 VAHASARPDV LRTALIRGAF DYQGQKCSAV SRAFIAHSVW QRMGDELLAK AAELRYGDIT
      361 DLSNYGGALI DQRAFVKNVD AIERAKGAAA VTVAVGGEYD DSEGYFVRPT VLLSDDPTDE
      421 SFVIEYFGPL LSVHVYPDER YEQILDVIDT GSRYALTGAV IADDRQAVLT ALDRLRFAAG
      481 NFYVNDKPTG AVVGRQPFGG ARGSGTNDKA GSPLNLLRWT SARSIKETFV AATDHIYPHM
      541 AVD
//