LOCUS SIT99818.1 578 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable fatty-acid-amp ligase fadd21 (fatty-acid-amp synthetase) (fatty-acid-amp synthase) protein. ACCESSION LT708304-1230 PROTEIN_ID SIT99818.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD21" /locus_tag="BQ2027_MB1217C" /note="Mb1217c, fadD21, len: 578 aa. Equivalent to Rv1185c, len: 578 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 578 aa overlap). Probable fadD21, fatty-acid-CoA synthetase (EC 6.2.1.-), highly similar to several from Mycobacteria e.g. NP_301895.1|NC_002677 possible acyl-CoA synthase from Mycobacterium leprae (579 aa); P71495|U75685 ACYL-COA SYNTHASE from Mycobacterium bovis (582 aa), FASTA scores: opt: 2388, E(): 0, (61.8% identity in 579 aa overlap); etc. SEEMS TO BELONG TO THE ATP-DEPENDENT AMP-BINDING ENZYME FAMILY. Protein product from Mb1217c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1217c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P63524" /db_xref="InterPro:IPR000873" /db_xref="InterPro:IPR040097" /db_xref="InterPro:IPR042099" /db_xref="UniProtKB/Swiss-Prot:P63524" BEGIN 1 MSDSSVLSLL RERAGLQPDD AAFTYIDYEQ DWAGITETLT WSEVFRRTRI VAHEVRRHCT 61 TGDRAVILAP QGLAYIAAFL GSMQAGAIAV PLSVPQIGSH DERVSAVLAD ASPSVILTTS 121 AVAEAVAEHI HRPNTNNVGP IIEIDSLDLT GNSPSFRVKD LPSAAYLQYT SGSTRAPAGV 181 MISHRNLQAN FQQLMSNYFG DRNGVAPPDT TIVSWLPFYH DMGLVLGIIA PILGGYRSEL 241 TSPLAFLQRP ARWLHSLANG SPSWSAAPNF AFELAVRKTT DADIEGLDLG NVLGITSGAE 301 RVHPNTLSRF CNRFAPYNFR EDMIRPSYGL AEATLYVASR NSGDKPEVVY FEPDKLSTGS 361 ANRCEPKTGT PLLSYGMPTS PTVRIVDPDT CIECPAGTIG EIWVKGDNVA EGYWNKPDET 421 RHTFGAMLVH PSAGTPDGSW LRTGDLGFLS EDEMFIVGRM KDMLIVYGRN HYPEDIESTV 481 QEITGGRVAA ISVPVDHTEK LVTVIELKLL GDSAGEAMDE LDVIKNNVTA AISRSHGLNV 541 ADLVLVPPGS IPTTTSGKIR RAACVEQYRL QQFTRLDG //