LOCUS SIT99817.1 359 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE EXPORTED PROTEIN protein. ACCESSION LT708304-1229 PROTEIN_ID SIT99817.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1216C" /note="Mb1216c, -, len: 359 aa. Equivalent to Rv1184c, len: 359 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 359 aa overlap). Possible exported protein with potential N-terminal signal sequence. Similar to several Mycobacterial hypothetical proteins e.g. Q49633|U00010) Protein B1170_F3_112 from M. leprae (391 aa), FASTA scores: opt: 1422, E(): 0, (62.7% identity in 338 aa overlap). Also similar to Rv3822, Rv3539, Rv1430, Rv0151c, etc. Protein product from Mb1216c detected using SWATH mass spectrometry. Mb1216c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR013228" /db_xref="UniProtKB/TrEMBL:A0A1R3XXL5" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MKRVIAGAFA VWLVGWAGGF GTAIAASEPA YPWAPGPPPS PSPVGDASTA KVVYALGGAR 61 MPGIPWYEYT NQAGSQYFPN AKHDLIDYPA GAAFSWWPTM LLPPGSHQDN MTVGVAVKDG 121 TNSLDNAIHH GTDPAAAVGL SQGSLVLDQE QARLANDPTA PAPDKLQFTT FGDPTGRHAF 181 GASFLARIFP PGSHIPIPFI EYTMPQQVDS QYDTNHVVTA YDGFSDFPDR PDNLLAVANA 241 AIGAAIAHTP IGFTGPGDVP PQNIRTTVNS RGATTTTYLV PVNHLPLTLP LRYLGMSDAE 301 VDQIDSVLQP QIDAAYARND NWFTRPVSVD PVRGLDPLTA PGSIVEGARG LLGSPAFGG //