LOCUS SIT99815.1 472 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED POLYKETIDE SYNTHASE ASSOCIATED PROTEIN PAPA3 protein. ACCESSION LT708304-1227 PROTEIN_ID SIT99815.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="papA3" /locus_tag="BQ2027_MB1214" /note="Mb1214, papA3, len: 472 aa. Equivalent to Rv1182, len: 472 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 472 aa overlap). Probable papA3, conserved polyketide synthase (PKS) associated protein, similar to other Mycobacterial hypothetical proteins e.g. Q49618|U00010 B1170_C1_180 from Mycobacterium leprae (471 aa), FASTA scores: opt: 2526, E(): 0, (75.6% identity in 471 aa overlap). Similar to other Mycobacterium tuberculosis hypothetical papA proteins; Rv3824c, Rv3820c, Rv1528c. Protein product from Mb1214 detected using SWATH mass spectrometry. Mb1214 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR001242" /db_xref="InterPro:IPR023213" /db_xref="UniProtKB/TrEMBL:A0A1R3XXY4" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MLRVGPLTIG TLDDWAPSTG STVSWRPSAV AHTKASQAPI SDVPVSYMQA QHIRGYCEQK 61 AKGLDYSRLM VVSCQQPGQC DIRAANYVIN AHLRRHDTYR SWFQYNGNGQ IIRRTIQDPA 121 DIEFVPVHHG ELTLPQIREI VQNTPDPLQW GCFRFGIVQG CDHFTFFASV DHVHVDAMIV 181 GVTLMEFHLM YAALVGGHAP LELPPAGSYD DFCRRQHTFS STLTVESPQV RAWTKFAEGT 241 NGSFPDFPLP LGDPSKPSDA DIVTVMMLDE EQTAQFESVC TAAGARFIGG VLACCGLAEH 301 ELTGTTTYYG LTPRDTRRTP ADAMTQGWFT GLIPITVPIA GSAFGDAARA AQTSFDSGVK 361 LAEVPYDRVV ELSSTLTMPR PNFPVVNFLD AGAAPLSVLL TAELTGTNIG VYSDGRYSYQ 421 LSIYVIRVEQ GTAVAVMFPD NPIARESVAR YLATLKSVFQ RVAESGQQQN VA //