LOCUS SIT99814.1 2085 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable
polyketide beta-ketoacyl synthase pks4 protein.
ACCESSION LT708304-1226
PROTEIN_ID SIT99814.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="pks3"
/locus_tag="BQ2027_MB1213"
/note="Mb1213, pks3, len: 2085 aa. Equivalent to Rv1180
and Rv1181, len: 488 aa and 1582 aa, from Mycobacterium
tuberculosis strain H37Rv, (100% identity in 488 aa
overlap and 99.9% identity in 1582 aa overlap). Probable
polyketide beta-ketoacyl synthase (EC 2.3.1.-), similar to
the N-terminus of many polyketide synthases e.g.
MCAS_MYCBO|Q02251 mycocerosic acid synthase from
mycobacterium bovis (2110 aa), FASTA scores: opt: 2115,
E(): 0, (66.5% identity in 472 aa overlap). Also similar
to, and same length as P96284|Z83858|MTCY24G1.02 M.
tuberculosis (496 aa), FASTA scores: opt: 1424, E(): 0,
(50.9% identity in 444 aa overlap). Contains possible
signal sequence and PS00013 Prokaryotic membrane
lipoprotein lipid attachment site, also PS00606
Beta-ketoacyl synthases active site. BELONGS TO THE
BETA-KETOACYL-ACP SYNTHASES FAMILY. Probable polyketide
synthase, similar to many e.g. MCAS_MYCBO|Q02251
mycocerosic acid synthase from mycobacterium bovis (2110
aa), FASTA scores: opt: 3518, E(): 0, (59.7% identity in
1614 aa overlap). Note that this similarity extends
upstream of the first initiation codon into the upstream
MTV005.16; however the stop codon at the end of MTV005.16
is present in at least 4 independent clones (BAC, cosmid
and pUC) from the genome. The two CDS's may represent
separate modules of the polyketide synthase.
REMARK-M.bovis-M.tuberculosis: In Mycobacterium
tuberculosis strain H37Rv, Rv1180 and Rv1181 exist as 2
genes. In Mycobacterium bovis, a single base transversion
(a-c) leads to a single product (similar to other
organisms). Protein product from Mb1213 detected using
shotgun mass spectrometry and SWATH mass spectrometry.
Mb1213 found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing."
/db_xref="GOA:A0A1R3XXM6"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR011032"
/db_xref="InterPro:IPR013149"
/db_xref="InterPro:IPR013154"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR016035"
/db_xref="InterPro:IPR016036"
/db_xref="InterPro:IPR016039"
/db_xref="InterPro:IPR018201"
/db_xref="InterPro:IPR020801"
/db_xref="InterPro:IPR020806"
/db_xref="InterPro:IPR020807"
/db_xref="InterPro:IPR020841"
/db_xref="InterPro:IPR020843"
/db_xref="InterPro:IPR032821"
/db_xref="InterPro:IPR036291"
/db_xref="InterPro:IPR036736"
/db_xref="InterPro:IPR042104"
/db_xref="UniProtKB/TrEMBL:A0A1R3XXM6"
BEGIN
1 MRTATATSVA VIGMACRLPG GIDSPQRLWE ALLRGDDLVG EIPADRWDAN VYYDPEPGVP
61 GRSVSRWGAF LDDVGGFDCD FFGLTEREAT AIDPQHRLLL EVSWEAIEHA GVDPATLAES
121 QTGVFVGLTH GDYELLSADC GAAEGPYGFT GTSNSFASGR VAYTLGLHGP AVTVDTACSS
181 GLTAVHQACR SLDDGESDLA LAGGVVVTLE PRKSVSGSLQ GMLSPTGRCH AFDEAADGFV
241 SGEGCVVLLL KRLPDAVRDG DRVLAIVRGT AANQDGRTVN IAAPSAQAQI AVYQQALAAA
301 GVEASTVGMV EAHGTGTPVG DPVEYASLAA VYGTEGPCAL TSVKTNFGHL QSASGPLGLM
361 KTILALRHGV VPQNLHFCRL PDQLAEIDTE LFVPQANTSW PDNTGQPRRA AVSSYGMSGT
421 NVHAILEQAP VSEPAASGPE LTPEAGGLAL FPVSATSAEQ LHVTAARLAD WVDQNGNAGS
481 RVSMRDLGYT LSCRRAHRPV RTVVTASSFD ELSAALRDVA GDQIPYQPAV GHDDRGPVWV
541 FSGQGSQWPG MGTELLVAEP VFAATVAAME PVIARESGFS VTEAMSAPQT VSGIDRVQPT
601 IFAVQVALAA ALKSYGVRPG AIIGHSLGEA AAAVVAGALS LHDGLRVICR RSRLMSRIAG
661 SGAMASVELP GQQVLSELAI RGISDVVLSV VASPTSTVVG GATQSIRDLV AAWEQQDVLA
721 REVAVDVASH TPQVDPILDE LLEVLAEVDP TAPEIPYYSA TLWDPRERPS FTGEYWVENL
781 RYTVRFAAAV QAALKDGYRV FGELAPHPLL TYAVEQNAAS LDMPIATLAA MRRGEQLPFG
841 LRGFVADVHN AGAKVDFSVQ YPDGRLVDAP LPSWTHRTLM LSREDSHRSH TGAVQAVHPL
901 LGAHVHLLEE PERHVWQAGV GTGAHPWLGD HRIHNVAAFP GAAYCEMALA AARTTLGELS
961 EVRDIKFEQT LLLDEQTVVS SAATIAAPGI LQFAVESHQE GEPARRASAM LHALEEMPQP
1021 PGYDTNALTA AHESSMSGEE LRKMFNSLGI QYGPAFSGLV AVHTARGAVT TVLAEVALPG
1081 AIRSQQSAYA SHPALLDACF QSVLVHPEVQ KATVGGLMLP VGVRRLRNYH STRSAHYCLA
1141 RVTSSSRAGE CEADLDVFDQ AGTVLLTVEG LRLAAGISEH ERANRVFDER LLTIEWERGE
1201 LPEVPQIDAG SWLLLSASEA DPLTAQLADA LNAVGAQSTS VASASDVAQL RSLLGGRLTG
1261 VVVVTGPPTG GLTQCGRDYV SQLVGIAREL AELPGEPPRL FVVTRSAASV LPSDLANLEQ
1321 AGLRGLMRVI DSEHPHLGAT AIDVDNDETV AALVASQLQS GSQEDETAWR NGIWYTARLR
1381 PGPLRPAERR TAVVEYRRDG MRLQIRTPGD LESLEFVTFD RVAPGPGEIE VAVTASSVNF
1441 ADVLVAFGRY PTFEGYRQQL GIDFAGVVTA VGPDVTEHRI GDHVGGMSAN GCWSTFVRCD
1501 ARLAVTLPPE LPVAAAAAVP TASATAWYAL HDLARICSDD KVLIHSGTGG VGQAAIAIAR
1561 AAGCEIFATA GSAQRRQLLH DMGVEHVYDS RSTEFAEQIR GDTDGYGVDV VLNSLPGAAQ
1621 RAGIELLAFG GRFVEIGKRD IYGDTRLGLF PFRRNLSLYA VDLALLTHSH PHTVRRLLKT
1681 VYQHTVEGTL PVPQTTHYPI HDAAVAIRLV GGAGHTGKVV LDVPRTGEGV AVVPPEQVRT
1741 SRPDGAYLVT GGLGGLGLFL AGELAAAGCG RIVLNSRSTP SPHATRVIER LRAAGADIQV
1801 ECGDIADAAT AHRVVAVATA SGLPVRGVLH AAAVVEDATL ANVTDELIDR CWAPKVHGAW
1861 NIHRATAAQP LEWFCLFSSA AALVGSPGQG AYAAANSWLD AFAHWRRAQG LPATSIAWGA
1921 WAEIGRATAL AEGTGAAIAP AEGARAFQTL LRYGRAYSGY APIMGTPWLT AFAQRSRFAE
1981 AFHATGQNQP ATGKFLAELG SLPREEWPRT VRRLVSDQIS LLLRRTIDPD RPLSDYGLDS
2041 LGNLELRTRI ETETGIRVSP TKITTVRGLA EHVCDELAAA QSAPV
//