LOCUS SIT99798.1 628 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE GTP-BINDING TRANSLATION ELONGATION FACTOR TYPA (TYROSINE PHOSPHORYLATED PROTEIN A) (GTP-BINDING PROTEIN) protein. ACCESSION LT708304-1210 PROTEIN_ID SIT99798.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="typA" /locus_tag="BQ2027_MB1197" /note="Mb1197, typA, len: 628 aa. Equivalent to Rv1165, len: 628 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 628 aa overlap). Possible typA (alternate gene name: bipA), GTP-binding translation elongation factor, similar to several e.g. P32132|TYPA_ECOLI|BIPA|B387 Escherichia coli (591 aa); YIHK_SYNY3|P72749 GTP-binding protein TYPA/BIPA homolog from synechocystis sp. (597 aa), FASTA scores: E(): 0, (46.9% identity in 610 aa overlap); and to elongation factor EF-G from many organims e.g. EFG_MICLU|P09952 micrococcus luteus (701 aa), FASTA scores: E(): 3e-24, (29.8% identity in 500 aa overlap). BELONGS TO THE GTP-BINDING ELONGATION FACTOR FAMILY, TYPA SUBFAMILY. Protein product from Mb1197 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1197 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXJ4" /db_xref="InterPro:IPR000640" /db_xref="InterPro:IPR000795" /db_xref="InterPro:IPR004161" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR006298" /db_xref="InterPro:IPR009000" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR035647" /db_xref="InterPro:IPR035651" /db_xref="InterPro:IPR042116" /db_xref="UniProtKB/TrEMBL:A0A1R3XXJ4" BEGIN 1 MPFRNVAIVA HVDHGKTTLV DAMLRQSGAL RERGELQERV MDTGDLEREK GITILAKNTA 61 VHRHHPDGTV TVINVIDTPG HADFGGEVER GLSMVDGVLL LVDASEGPLP QTRFVLRKAL 121 AAHLPVILVV NKTDRPDARI AEVVDASHDL LLDVASDLDD EAAAAAEHAL GLPTLYASGR 181 AGVASTTAPP DGQVPDGTNL DPLFEVLEKH VPPPKGEPDA PLQALVTNLD ASTFLGRLAL 241 IRIYNGRIRK GQQVAWIRQV DGQQTVTTAK ITELLATEGV ERKPTDAAVA GDIVAVAGLP 301 EIMIGDTLAA SANPVALPRI TVDEPAISVT IGTNTSPLAG KVGGHKLTAR MVRSRLDAEL 361 VGNVSIRVVD IGAPDAWEVQ GRGELALAVL VEQMRREGFE LTVGKPQVVT KTIDGTLHEP 421 FESMTVDCPE EYIGAVTQLM AARKGRMVEM ANHTTGWVRM DFVVPSRGLI GWRTDFLTET 481 RGSGVGHAVF DGYRPWAGEI RARHTGSLVS DRAGAITPFA LLQLADRGQF FVEPGQQTYE 541 GMVVGINPRP EDLDINVTRE KKLTNMRSST ADVIETLAKP LQLDLERAME LCAPDECVEV 601 TPEIVRIRKV ELAAAARARS RARTKARG //