LOCUS SIT99796.1 201 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE RESPIRATORY NITRATE REDUCTASE (DELTA CHAIN) NARJ protein. ACCESSION LT708304-1208 PROTEIN_ID SIT99796.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="narJ" /locus_tag="BQ2027_MB1195" /note="Mb1195, narJ, len: 201 aa. Equivalent to Rv1163, len: 201 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 201 aa overlap). Probable narJ, respiratory nitrate reductase delta chain (EC 1.7.99.4). Similar to others e.g. P42178|NARJ_BACSU NITRATE REDUCTASE DELTA CHAIN from Bacillus subtilis (184 aa), FASTA scores: opt: 254, E(): 1.9e-10, (31.8% identity in 179 aa overlap); etc. Strong similarity to region from aa 260 -410 of Rv1736c|MTCY04C12.21c|NARX PROBABLE NITRATE REDUCTASE from Mycobacterium tuberculosis (64.8% identity in 159 aa overlap). Protein product from Mb1195 detected using SWATH mass spectrometry. Mb1195 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXJ7" /db_xref="InterPro:IPR003765" /db_xref="InterPro:IPR020945" /db_xref="InterPro:IPR036411" /db_xref="UniProtKB/TrEMBL:A0A1R3XXJ7" BEGIN 1 MWQSASLLLA YPDDGLAERL HMVDALRAHQ TGPAAALLGR TVAELRALAP MAAAAQYVET 61 FDMRRRSTMY LTYWTAGDTR NRGREMLAFA TAYRDAGVKP PRTEAPDYLP VVLEFAATVD 121 PEAGRRLLTE HRVPIDVLRG ALADAKSPYE YTVAAICETL PAATNQEVRR AQRLAQSGPP 181 AEAVGLQPFT LTVPPKRAEG A //