LOCUS SIT99776.1 250 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE protein. ACCESSION LT708304-1188 PROTEIN_ID SIT99776.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1176" /note="Mb1176, -, len: 250 aa. Equivalent to Rv1144, len: 250 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 250 aa overlap). Probable short-chain dehydrogenase/reductase (EC 1.-.-.-), highly similar to various dehydrogenases e.g. NP_104056.1|NC_002678 3-hydroxyacyl-CoA dehydrogenase type II from Mesorhizobium loti (253 aa); NP_251244.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (255 aa); AAK15008.1|AF233685_1|AF233685 short chain L-3-hydroxyacyl-CoA dehydrogenase from Mus musculus (261 aa); HSU73514|g1778354|XH98G2 human short-chain alcohol dehydrogenase from Homo sapiens (261 aa), FASTA scores: opt: 875, E(): 0, (60.1% identity in 253 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. BELONGS TO THE SHORT-CHAIN DEHYDROGENASES/REDUCTASES (SDR) FAMILY. Protein product from Mb1176 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1176 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXH8" /db_xref="InterPro:IPR002347" /db_xref="InterPro:IPR020904" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3XXH8" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MKTKDAVAVV TGGASGLGLA TTKRLLDAGA QVVVVDLRGD DVVGGLGDRA RFAQADVTDE 61 AAVSNALELA DSLGPVRVVV NCAGTGNAIR VLSRDGVFPL AAFRKIVDIN LVGTFNVLRL 121 GAERIAKTEP IGEERGVIIN TASVAAFDGQ IGQAAYSASK GGVVGMTLPI ARDLASKLIR 181 VVTIAPGLFD TPLLASLPAE AKASLGQQVP HPSRLGNPDE YGALVLHIIE NPMLNGEVIR 241 LDGAIRMAPR //