LOCUS       SIT99773.1               268 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ENOYL-COA HYDRATASE ECHA11 (ENOYL HYDRASE) (UNSATURATED
            ACYL-COA HYDRATASE) (CROTONASE) protein.
ACCESSION   LT708304-1185
PROTEIN_ID  SIT99773.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="echA11"
                     /locus_tag="BQ2027_MB1173C"
                     /note="Mb1173c, echA11, len: 268 aa. Equivalent to
                     Rv1141c, len: 268 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (100% identity in 268 aa overlap). Probable
                     echA11, enoyl-CoA hydratase (EC 4.2.1.17), similar to
                     others e.g. P24162|ECHH_RHOCA PROBABLE ENOYL-COA HYDRATASE
                     from Rhodobacter capsulatus (257 aa); CAA66096.1|X97452
                     enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA
                     scores: opt: 513, E(): 1e-25, (36.1% identity in 249 aa
                     overlap); etc. Also similarity with naphthoate synthases.
                     Also highly similar to downstream ORF
                     Rv1142c|MTCI65.09|echA10 PROBABLE ENOYL-COA HYDRATASE from
                     Mycobacterium tuberculosis (268 aa), FASTA scores: opt:
                     1225, E(): 0, (72.3% identity in 267 aa overlap). Protein
                     product from Mb1173c detected using SWATH mass
                     spectrometry. Mb1173c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3XYF7"
                     /db_xref="InterPro:IPR001753"
                     /db_xref="InterPro:IPR014748"
                     /db_xref="InterPro:IPR029045"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XYF7"
BEGIN
        1 MPDSGIAALT PVTGLNVTLT DRVLSVRINR PSSLNSLTVP ILTGIADTLE RAAADPVVKV
       61 VRLGGVGRGF SSGVSMSVDD VWGGGPPTAI VEEANRAVRA VAALPHPVVA VVQGPAVGVA
      121 VSLALACDFI LASDSAFFML ANTKVALMPD GGASALVAAA TGRIRAMRLA LLAEQLPARE
      181 ALAWGLISAV YPDSDFEAEV DKVISRLLAG PALAFAQAKN AINAAALTEL EPTFARELDG
      241 QEVLLRTHDF AEGAAAFLQR RTPNFTGS
//