LOCUS SIT99773.1 268 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ENOYL-COA HYDRATASE ECHA11 (ENOYL HYDRASE) (UNSATURATED ACYL-COA HYDRATASE) (CROTONASE) protein. ACCESSION LT708304-1185 PROTEIN_ID SIT99773.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="echA11" /locus_tag="BQ2027_MB1173C" /note="Mb1173c, echA11, len: 268 aa. Equivalent to Rv1141c, len: 268 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 268 aa overlap). Probable echA11, enoyl-CoA hydratase (EC 4.2.1.17), similar to others e.g. P24162|ECHH_RHOCA PROBABLE ENOYL-COA HYDRATASE from Rhodobacter capsulatus (257 aa); CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 513, E(): 1e-25, (36.1% identity in 249 aa overlap); etc. Also similarity with naphthoate synthases. Also highly similar to downstream ORF Rv1142c|MTCI65.09|echA10 PROBABLE ENOYL-COA HYDRATASE from Mycobacterium tuberculosis (268 aa), FASTA scores: opt: 1225, E(): 0, (72.3% identity in 267 aa overlap). Protein product from Mb1173c detected using SWATH mass spectrometry. Mb1173c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XYF7" /db_xref="InterPro:IPR001753" /db_xref="InterPro:IPR014748" /db_xref="InterPro:IPR029045" /db_xref="UniProtKB/TrEMBL:A0A1R3XYF7" BEGIN 1 MPDSGIAALT PVTGLNVTLT DRVLSVRINR PSSLNSLTVP ILTGIADTLE RAAADPVVKV 61 VRLGGVGRGF SSGVSMSVDD VWGGGPPTAI VEEANRAVRA VAALPHPVVA VVQGPAVGVA 121 VSLALACDFI LASDSAFFML ANTKVALMPD GGASALVAAA TGRIRAMRLA LLAEQLPARE 181 ALAWGLISAV YPDSDFEAEV DKVISRLLAG PALAFAQAKN AINAAALTEL EPTFARELDG 241 QEVLLRTHDF AEGAAAFLQR RTPNFTGS //