LOCUS SIT99740.1 335 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE LYTB-RELATED PROTEIN LYTB2 protein. ACCESSION LT708304-1152 PROTEIN_ID SIT99740.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="lytB2" /locus_tag="BQ2027_MB1140" /note="Mb1140, lytB2, len: 335 aa. Equivalent to Rv1110, len: 335 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 335 aa overlap). Probable lytB2, LytB-related protein, equivalent to AL049491|MLCB1222_3 from Mycobacterium leprae (335 aa), FASTA score: (82.9% identity in 333 aa overlap). Also similar to LytB proteins from many bacteria (appears to have N-terminal extension) e.g. P22565|LYTB_ECOLI|B0029|Z0034|ECS0032 LYTB PROTEIN from Escherichia coli strains K12 and O157:H7 (316 aa), FASTA scores: opt: 1041, E():0, (52.4% identity in 309 aa overlap); etc. Also very similar to another LytB-related protein from Mycobacterium tuberculosis: LytB1|Rv3382c|MTV004.40c (329 aa), FASTA scores: opt: 975, E(): 0, (51.3% identity in 312 aa overlap). BELONGS TO THE LYTB FAMILY. Protein product from Mb1140 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1140 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A5I1" /db_xref="InterPro:IPR003451" /db_xref="UniProtKB/Swiss-Prot:P0A5I1" BEGIN 1 MVPTVDMGIP GASVSSRSVA DRPNRKRVLL AEPRGYCAGV DRAVETVERA LQKHGPPVYV 61 RHEIVHNRHV VDTLAKAGAV FVEETEQVPE GAIVVFSAHG VAPTVHVSAS ERNLQVIDAT 121 CPLVTKVHNE ARRFARDDYD ILLIGHEGHE EVVGTAGEAP DHVQLVDGVD AVDQVTVRDE 181 DKVVWLSQTT LSVDETMEIV GRLRRRFPKL QDPPSDDICY ATQNRQVAVK AMAPECELVI 241 VVGSRNSSNS VRLVEVALGA GARAAHLVDW ADDIDSAWLD GVTTVGVTSG ASVPEVLVRG 301 VLERLAECGY DIVQPVTTAN ETLVFALPRE LRSPR //