LOCUS SIT99738.1 415 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE EXODEOXYRIBONUCLEASE VII (LARGE SUBUNIT) XSEA (EXONUCLEASE VII LARGE SUBUNIT) protein. ACCESSION LT708304-1150 PROTEIN_ID SIT99738.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="xseA" /locus_tag="BQ2027_MB1138C" /note="Mb1138c, xseA, len: 415 aa. Equivalent to Rv1108c, len: 415 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 415 aa overlap). Probable xseA, exodeoxyribonuclease VII large subunit (EC 3.1.11.6) (see first citation below). Equivalent to AL049491|MLCB1222_5 Mycobacterium leprae (428 aa) (81.5% identity in 411 aa overlap). Similar to many e.g. P04994|EX7L_ECOLI exodeoxyribonuclease large subunit from Escherichia coli (456 aa), FASTA scores: opt: 581, E(): 1.6 e-30, (30.8% identity in 425 aa overlap); also similar to the exodeoxyribonuclease in Bacillus subtilis, H. influenzae and H. pylori. TBparse score is 0.890. BELONGS TO THE XSEA FAMILY. Protein product from Mb1138c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1138c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P67448" /db_xref="InterPro:IPR003753" /db_xref="InterPro:IPR020579" /db_xref="InterPro:IPR025824" /db_xref="UniProtKB/Swiss-Prot:P67448" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTQNSAENPF PVRAVAIRVA GWIDKLGAVW VEGQLAQITM RPDAKTVFMV LRDPAADMSL 61 TVTCSRDLVL SAPVKLAEGV QVVVCGKPSF YTGRGTFSLR LSEIRAVGIG ELLARIDRLR 121 RLLDAEGLFD PRLKRPIPYL PNMIGLITGR ASAAERDVTT VASARWPAAR FAVRNVAVQG 181 PNAVGQIVEA LRELDRDPDV DVIVLARGGG SVEDLLPFSD ETLCRAIAAC RTPVVSAVGH 241 EPDNPLCDLV VDLRAATPTD AAKKVVPDTA AEQRLIDDLR RRSAQALRNW VSREQRAVAQ 301 LRSRPVLADP MTMVSVRAEE VHRARSTLRR NLTLMVAAET ERIGHLAARL ATLGPAATLA 361 RGYAIVQTVA QTGPEGGSEP QVLRSVHDAP EGTKLRVRVA DGALAAVSEG QTNGL //