LOCUS       SIT99728.1               474 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            FUMARASE FUM (Fumarate hydratase) protein.
ACCESSION   LT708304-1140
PROTEIN_ID  SIT99728.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fum"
                     /locus_tag="BQ2027_MB1128C"
                     /note="Mb1128c, fum, len: 474 aa. Equivalent to Rv1098c,
                     len: 474 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 474 aa overlap). Probable fum, fumarase
                     (EC 4.2.1.2). Equivalent to AL049491|MLCB1222_11
                     Mycobacterium leprae (474 aa) (89.5 % identity in 467 aa
                     overlap). Similar to many e.g. P14408|FUMH_RAT FUMARATE
                     HYDRATASE, MITOCHONDRIAL PRECURSOR from Rattus norvegicus
                     (507 aa), FASTA scores: opt: 1427, E(): 0, (52.3% identity
                     in 461 aa overlap); and P05042|FUMC_ECOLI Fumarate
                     hydratase class II from Escherichia coli (467 aa), FASTA
                     scores: opt: 1355, E(): 0, (50.2% identity in 444 aa
                     overlap). Contains PS00163 Fumarate lyases signature.
                     TBparse score is 0.886. Protein product from Mb1128c
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb1128c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:Q7U0N6"
                     /db_xref="InterPro:IPR000362"
                     /db_xref="InterPro:IPR005677"
                     /db_xref="InterPro:IPR008948"
                     /db_xref="InterPro:IPR018951"
                     /db_xref="InterPro:IPR020557"
                     /db_xref="InterPro:IPR022761"
                     /db_xref="InterPro:IPR024083"
                     /db_xref="UniProtKB/Swiss-Prot:Q7U0N6"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MAVDADSANY RIEHDTMGEV RVPAKALWRA QTQRAVENFP ISGRGLERTQ IRALGLLKGA
       61 CAQVNSDLGL LAPEKADAII AAAAEIADGQ HDDQFPIDVF QTGSGTSSNM NTNEVIASIA
      121 AKGGVTLHPN DDVNMSQSSN DTFPTATHIA ATEAAVAHLI PALQQLHDAL AAKALDWHTV
      181 VKSGRTHLMD AVPVTLGQEF SGYARQIEAG IERVRACLPR LGELAIGGTA VGTGLNAPDD
      241 FGVRVVAVLV AQTGLSELRT AANSFEAQAA RDGLVEASGA LRTIAVSLTK IANDIRWMGS
      301 GPLTGLAEIQ LPDLQPGSSI MPGKVNPVLP EAVTQVAAQV IGNDAAIAWG GANGAFELNV
      361 YIPMMARNIL ESFKLLTNVS RLFAQRCIAG LTANVEHLRR LAESSPSIVT PLNSAIGYEE
      421 AAAVAKQALK ERKTIRQTVI DRGLIGDRLS IEDLDRRLDV LAMAKAEQLD SDRL
//