LOCUS SIT99728.1 474 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE FUMARASE FUM (Fumarate hydratase) protein. ACCESSION LT708304-1140 PROTEIN_ID SIT99728.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fum" /locus_tag="BQ2027_MB1128C" /note="Mb1128c, fum, len: 474 aa. Equivalent to Rv1098c, len: 474 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 474 aa overlap). Probable fum, fumarase (EC 4.2.1.2). Equivalent to AL049491|MLCB1222_11 Mycobacterium leprae (474 aa) (89.5 % identity in 467 aa overlap). Similar to many e.g. P14408|FUMH_RAT FUMARATE HYDRATASE, MITOCHONDRIAL PRECURSOR from Rattus norvegicus (507 aa), FASTA scores: opt: 1427, E(): 0, (52.3% identity in 461 aa overlap); and P05042|FUMC_ECOLI Fumarate hydratase class II from Escherichia coli (467 aa), FASTA scores: opt: 1355, E(): 0, (50.2% identity in 444 aa overlap). Contains PS00163 Fumarate lyases signature. TBparse score is 0.886. Protein product from Mb1128c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1128c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7U0N6" /db_xref="InterPro:IPR000362" /db_xref="InterPro:IPR005677" /db_xref="InterPro:IPR008948" /db_xref="InterPro:IPR018951" /db_xref="InterPro:IPR020557" /db_xref="InterPro:IPR022761" /db_xref="InterPro:IPR024083" /db_xref="UniProtKB/Swiss-Prot:Q7U0N6" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MAVDADSANY RIEHDTMGEV RVPAKALWRA QTQRAVENFP ISGRGLERTQ IRALGLLKGA 61 CAQVNSDLGL LAPEKADAII AAAAEIADGQ HDDQFPIDVF QTGSGTSSNM NTNEVIASIA 121 AKGGVTLHPN DDVNMSQSSN DTFPTATHIA ATEAAVAHLI PALQQLHDAL AAKALDWHTV 181 VKSGRTHLMD AVPVTLGQEF SGYARQIEAG IERVRACLPR LGELAIGGTA VGTGLNAPDD 241 FGVRVVAVLV AQTGLSELRT AANSFEAQAA RDGLVEASGA LRTIAVSLTK IANDIRWMGS 301 GPLTGLAEIQ LPDLQPGSSI MPGKVNPVLP EAVTQVAAQV IGNDAAIAWG GANGAFELNV 361 YIPMMARNIL ESFKLLTNVS RLFAQRCIAG LTANVEHLRR LAESSPSIVT PLNSAIGYEE 421 AAAVAKQALK ERKTIRQTVI DRGLIGDRLS IEDLDRRLDV LAMAKAEQLD SDRL //