LOCUS SIT99726.1 291 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE GLYCOSYL HYDROLASE protein. ACCESSION LT708304-1138 PROTEIN_ID SIT99726.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1126" /note="Mb1126, -, len: 291 aa. Equivalent to Rv1096, len: 291 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 291 aa overlap). Possible glycosyl hydrolase (EC 3.-.-.-), possibly a deacetylase or esterase. Equivalent to AL049491|MLCB1222_13 Mycobacterium leprae (291 aa) (81.3% identity in 289 aa overlap). Similar at the C-terminus of enzymes involved in carbohydrate degradation including Z99110|BSUB0007_92 endo-1,4-beta-xylanase homolog yjeA from Bacillus subtilis (467 aa), FASTA scores: opt: 418, E(): 2.6e-17, (38.6% identity in 184 aa overlap); M64552|STMXLNB_2 acetyl-xylan esterase from Streptomyces lividans (335 aa), FASTA scores: opt: 371, E(): 1.1e-14, (31.6% identity in 237 aa overlap); NP_345933.1|NC_003028 peptidoglycan N-acetylglucosamine deacetylase A from Streptococcus pneumoniae (463 aa); etc. Has possible N-terminal signal sequence with TMhelix at aa 13-31. Protein product from Mb1126 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1126 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXC7" /db_xref="InterPro:IPR002509" /db_xref="InterPro:IPR011330" /db_xref="UniProtKB/TrEMBL:A0A1R3XXC7" BEGIN 1 MPKRPDNQTW RYWRTVTGVV VAGAVLVVGG LSGRVTRAEN LSCSVIKCVA LTFDDGPGPY 61 TDRLLHILTD NDAKATFFLI GNKVAANPAG ARRIADAGME IGSHTWEHPN MTTIPPEDIP 121 GQFSRANDVI AAATGRTPTL YRPAGGLSND AVRQAAAKVG QAEILWDVIP FDWINDSNTA 181 ATRHMLMTQI KPGSVVLFHD TYSSTVDVVY QFIPVLKANG YRLVTVSELL GPRAPGSSYG 241 SRENGPPVNE LRDIPASEIP PLPNTSSPKP MSNFPITDIA GQNSGGPNNG A //