LOCUS SIT99710.1 288 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Mycothiol conjugate amidase Mca (Mycothiol S-conjugate amidase) protein. ACCESSION LT708304-1122 PROTEIN_ID SIT99710.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mca" /locus_tag="BQ2027_MB1111" /note="Mb1111, mca, len: 288 aa. Equivalent to Rv1082, len: 288 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 288 aa overlap). mca, mycothiol conjugate amidase (see citation below), equivalent to NP_302547.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (290 aa), FASTA scores: opt: 1737, E(): 0, (86.4% identity in 287 aa overlap); and similar to Q54358|X79146 lmbE protein from Streptomyces lincolnensis (270 aa). Also similar to Rv1170|MTV005.06|MSHB GlcNAc-Ins deacetylase from Mycobacterium tuberculosis (303 aa), FASTA scores: opt: 411, E(): 9.4e-20, (35.8% identity in 299 aa overlap). Protein product from Mb1111 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1111 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXA8" /db_xref="InterPro:IPR003737" /db_xref="InterPro:IPR017811" /db_xref="InterPro:IPR024078" /db_xref="UniProtKB/TrEMBL:A0A1R3XXA8" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSELRLMAVH AHPDDESSKG AATLARYADE GHRVLVVTLT GGERGEILNP AMDLPDVHGR 61 IAEIRRDEMT KAAEILGVEH TWLGFVDSGL PKGDLPPPLP DDCFARVPLE VSTEALVRVV 121 REFRPHVMTT YDENGGYPHP DHIRCHQVSV AAYEAAGDFC RFPDAGEPWT VSKLYYVHGF 181 LRERMQMLQD EFARHGQRGP FEQWLAYWDP DHDFLTSRVT TRVECSKYFS QRDDALRAHA 241 TQIDPNAEFF AAPLAWQERL WPTEEFELAR SRIPARPPET ELFAGIEP //