LOCUS       SIT99702.1               405 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            BETA-KETOACYL CoA THIOLASE FADA3 protein.
ACCESSION   LT708304-1114
PROTEIN_ID  SIT99702.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fadA3"
                     /locus_tag="BQ2027_MB1103C"
                     /note="Mb1103c, fadA3, len: 405 aa. Equivalent to Rv1074c,
                     len: 405 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 405 aa overlap). Probable fadA3,
                     beta-ketoacyl CoA thiolase (EC 2.3.1.-), highly similar to
                     many involved in beta-oxidation e.g. CAB89028.1|AL353870
                     beta-ketoadipyl-CoA thiolase from Streptomyces coelicolor
                     (395 aa); P77525|PAAJ_ECOLI probable beta-ketoadipyl CoA
                     thiolase from Escherichia coli (401 aa), FASTA scores:
                     opt: 1034, E(): 5.4e-56, (43.5% identity in 416 aa
                     overlap) and X97452 acetyl-CoA acetyltransferase
                     (thiolase) from Escherichia coli (401 aa), FASTA scores:
                     opt: 1043, E(): 0, (43.4% identity in 415 aa overlap);
                     Q43935|CATF_ACICA beta-ketoadipyl CoA thiolase from
                     Acinetobacter calcoaceticus (401 aa), FASTA scores: opt:
                     992, E(): 0, (41.5% identity in 415 aa overlap); etc.
                     Contains PS00737 Thiolases signature 2, and PS00445 FGGY
                     family of carbohydrate kinases signature 2, although this
                     is probably fortuitous. BELONGS TO THE THIOLASE FAMILY.
                     Protein product from Mb1103c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb1103c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3XZD9"
                     /db_xref="InterPro:IPR002155"
                     /db_xref="InterPro:IPR016039"
                     /db_xref="InterPro:IPR020613"
                     /db_xref="InterPro:IPR020616"
                     /db_xref="InterPro:IPR020617"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XZD9"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MPEAVIVSTA RSPIGRAMKG SLVGMRPDDL AVQMVRAALD KVPALNPHQI DDLMMGCGLP
       61 GGESGFNIAR VVAVALGYDF LPGTTVNRYC SSSLQTTRMA FHAIKAGEGD AFISAGVETV
      121 SRFAKGNSDS WPDTKNPLFD GAQERSAAAA AGADEWHDPR TDQKLPDIYI AMGQTAENVA
      181 IMTGISREEQ DRWGVRSQNR AEEAIKNGFF EREITPVTLP DGTTVSTDDG PRPGTTYEKV
      241 SELKPAFRPN GTVTAGNACP LNDGAAAVVI TSDTKAKELG LTPLARIVST GVSGLSPEIM
      301 GLGPIEASKK ALERAGMAIT DIDLVEINEA FAVQVLGSAR ELGIDEDKLN ISGGAIALGH
      361 PFGMTGARIT TTLLNNLQTY DKTFGLETMC VGGGQGMAMV IERLA
//