LOCUS SIT99702.1 405 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE BETA-KETOACYL CoA THIOLASE FADA3 protein. ACCESSION LT708304-1114 PROTEIN_ID SIT99702.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadA3" /locus_tag="BQ2027_MB1103C" /note="Mb1103c, fadA3, len: 405 aa. Equivalent to Rv1074c, len: 405 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 405 aa overlap). Probable fadA3, beta-ketoacyl CoA thiolase (EC 2.3.1.-), highly similar to many involved in beta-oxidation e.g. CAB89028.1|AL353870 beta-ketoadipyl-CoA thiolase from Streptomyces coelicolor (395 aa); P77525|PAAJ_ECOLI probable beta-ketoadipyl CoA thiolase from Escherichia coli (401 aa), FASTA scores: opt: 1034, E(): 5.4e-56, (43.5% identity in 416 aa overlap) and X97452 acetyl-CoA acetyltransferase (thiolase) from Escherichia coli (401 aa), FASTA scores: opt: 1043, E(): 0, (43.4% identity in 415 aa overlap); Q43935|CATF_ACICA beta-ketoadipyl CoA thiolase from Acinetobacter calcoaceticus (401 aa), FASTA scores: opt: 992, E(): 0, (41.5% identity in 415 aa overlap); etc. Contains PS00737 Thiolases signature 2, and PS00445 FGGY family of carbohydrate kinases signature 2, although this is probably fortuitous. BELONGS TO THE THIOLASE FAMILY. Protein product from Mb1103c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1103c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XZD9" /db_xref="InterPro:IPR002155" /db_xref="InterPro:IPR016039" /db_xref="InterPro:IPR020613" /db_xref="InterPro:IPR020616" /db_xref="InterPro:IPR020617" /db_xref="UniProtKB/TrEMBL:A0A1R3XZD9" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MPEAVIVSTA RSPIGRAMKG SLVGMRPDDL AVQMVRAALD KVPALNPHQI DDLMMGCGLP 61 GGESGFNIAR VVAVALGYDF LPGTTVNRYC SSSLQTTRMA FHAIKAGEGD AFISAGVETV 121 SRFAKGNSDS WPDTKNPLFD GAQERSAAAA AGADEWHDPR TDQKLPDIYI AMGQTAENVA 181 IMTGISREEQ DRWGVRSQNR AEEAIKNGFF EREITPVTLP DGTTVSTDDG PRPGTTYEKV 241 SELKPAFRPN GTVTAGNACP LNDGAAAVVI TSDTKAKELG LTPLARIVST GVSGLSPEIM 301 GLGPIEASKK ALERAGMAIT DIDLVEINEA FAVQVLGSAR ELGIDEDKLN ISGGAIALGH 361 PFGMTGARIT TTLLNNLQTY DKTFGLETMC VGGGQGMAMV IERLA //