LOCUS SIT99650.1 429 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ENOLASE ENO protein. ACCESSION LT708304-1062 PROTEIN_ID SIT99650.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="eno" /locus_tag="BQ2027_MB1051" /note="Mb1051, eno, len: 429 aa. Equivalent to Rv1023, len: 429 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 429 aa overlap). Probable eno, enolase (EC 4.2.1.11), highly similar to others e.g. ENO_ECOLI|P08324 enolase from Escherichia coli (431 aa), FASTA scores: opt: 1487, E(): 0, (55.5% identity in 422 aa overlap); etc. MAGNESIUM IS REQUIRED FOR CATALYSIS AND FOR STABILIZING THE DIMER. BELONGS TO THE ENOLASE FAMILY. Protein product from Mb1051 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1051 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:Q7U0U6" /db_xref="InterPro:IPR000941" /db_xref="InterPro:IPR020809" /db_xref="InterPro:IPR020810" /db_xref="InterPro:IPR020811" /db_xref="InterPro:IPR029017" /db_xref="InterPro:IPR036849" /db_xref="UniProtKB/Swiss-Prot:Q7U0U6" BEGIN 1 MPIIEQVGAR EILDSRGNPT VEVEVALIDG TFARAAVPSG ASTGEHEAVE LRDGGDRYGG 61 KGVQKAVQAV LDEIGPAVIG LNADDQRLVD QALVDLDGTP DKSRLGGNAI LGVSLAVAKA 121 AADSAELPLF RYVGGPNAHI LPVPMMNILN GGAHADTAVD IQEFMVAPIG APSFVEALRW 181 GAEVYHALKS VLKKEGLSTG LGDEGGFAPD VAGTTAALDL ISRAIESAGL RPGADVALAL 241 DAAATEFFTD GTGYVFEGTT RTADQMTEFY AGLLGAYPLV SIEDPLSEDD WDGWAALTAS 301 IGDRVQIVGD DIFVTNPERL EEGIERGVAN ALLVKVNQIG TLTETLDAVT LAHHGGYRTM 361 ISHRSGETED TMIADLAVAI GSGQIKTGAP ARSERVAKYN QLLRIEEALG DAARYAGDLA 421 FPRFACETK //