LOCUS       SIT99650.1               429 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ENOLASE ENO protein.
ACCESSION   LT708304-1062
PROTEIN_ID  SIT99650.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="eno"
                     /locus_tag="BQ2027_MB1051"
                     /note="Mb1051, eno, len: 429 aa. Equivalent to Rv1023,
                     len: 429 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 429 aa overlap). Probable eno, enolase
                     (EC 4.2.1.11), highly similar to others e.g.
                     ENO_ECOLI|P08324 enolase from Escherichia coli (431 aa),
                     FASTA scores: opt: 1487, E(): 0, (55.5% identity in 422 aa
                     overlap); etc. MAGNESIUM IS REQUIRED FOR CATALYSIS AND FOR
                     STABILIZING THE DIMER. BELONGS TO THE ENOLASE FAMILY.
                     Protein product from Mb1051 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb1051 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:Q7U0U6"
                     /db_xref="InterPro:IPR000941"
                     /db_xref="InterPro:IPR020809"
                     /db_xref="InterPro:IPR020810"
                     /db_xref="InterPro:IPR020811"
                     /db_xref="InterPro:IPR029017"
                     /db_xref="InterPro:IPR036849"
                     /db_xref="UniProtKB/Swiss-Prot:Q7U0U6"
BEGIN
        1 MPIIEQVGAR EILDSRGNPT VEVEVALIDG TFARAAVPSG ASTGEHEAVE LRDGGDRYGG
       61 KGVQKAVQAV LDEIGPAVIG LNADDQRLVD QALVDLDGTP DKSRLGGNAI LGVSLAVAKA
      121 AADSAELPLF RYVGGPNAHI LPVPMMNILN GGAHADTAVD IQEFMVAPIG APSFVEALRW
      181 GAEVYHALKS VLKKEGLSTG LGDEGGFAPD VAGTTAALDL ISRAIESAGL RPGADVALAL
      241 DAAATEFFTD GTGYVFEGTT RTADQMTEFY AGLLGAYPLV SIEDPLSEDD WDGWAALTAS
      301 IGDRVQIVGD DIFVTNPERL EEGIERGVAN ALLVKVNQIG TLTETLDAVT LAHHGGYRTM
      361 ISHRSGETED TMIADLAVAI GSGQIKTGAP ARSERVAKYN QLLRIEEALG DAARYAGDLA
      421 FPRFACETK
//