LOCUS SIT99645.1 495 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable UDP-N-acetylglucosamine pyrophosphorylase glmU protein. ACCESSION LT708304-1057 PROTEIN_ID SIT99645.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="glmU" /locus_tag="BQ2027_MB1046C" /note="Mb1046c, glmU, len: 495 aa. Equivalent to Rv1018c, len: 495 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 495 aa overlap). Probable glmU, UDP-n-acetylglucosamine pyrophosphorylase (EC 2.7.7.23), similar to GCAD_BACSU|P14192 UDP-n-acetylglucosamine pyrophosphorylase (456 aa), FASTA scores: opt: 1150, E(): 0, (40.0% identity in 453 aa overlap). Similar to various Mycobacterium tuberculosis sugar-phosphate transferases e.g. Rv0334, Rv1213, Rv3264c, etc. Protein product from Mb1046c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1046c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7VF00" /db_xref="InterPro:IPR001451" /db_xref="InterPro:IPR005882" /db_xref="InterPro:IPR011004" /db_xref="InterPro:IPR025877" /db_xref="InterPro:IPR029044" /db_xref="InterPro:IPR038009" /db_xref="UniProtKB/Swiss-Prot:Q7VF00" BEGIN 1 MTFPGDTAVL VLAAGPGTRM RSDTPKVLHT LAGRSMLSHV LHAIAKLAPQ RLIVVLGHDH 61 QRIAPLVGEL ADTLGRTIDV ALQDRPLGTG HAVLCGLSAL PDDYAGNVVV TSGDTPLLDA 121 DTLADLIATH RAVSAAVTVL TTTLDDPFGY GRILRTQDHG VMAIVEQTDA TPSQREIREV 181 NAGVYAFDIA ALRSALSRLS SNNAQQELYL TDVIAILRSD GQTVHASHVD DSALVAGVNN 241 RVQLAQLASE LNRRVVAAHQ LAGVTVVDPA TTWIDVDVTI GRDTVIHPGT QLLGRTQIGG 301 RCVVGPDTTL TDVAVGDGAS VVRTHGSSSS IGDGAAVGPF TYLRPGTALG ADGKLGAFVE 361 VKNSTIGTGT KVPHLTYVGD ADIGEYSNIG ASSVFVNYDG TSKRRTTVGS HVRTGSDTMF 421 VAPVTIGDGA YTGAGTVVRE DVPPGALAVS AGPQRNIENW VQRKRPGSPA AQASKRASEM 481 ACQQPTQPPD ADQTP //