LOCUS       SIT99638.1               306 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 Probable
            4-diphosphocytidyl-2-C-methyl-D- erythritol kinase ISPE
            (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol
            kinase) protein.
ACCESSION   LT708304-1050
PROTEIN_ID  SIT99638.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="ispE"
                     /locus_tag="BQ2027_MB1038"
                     /note="Mb1038, ispE, len: 306 aa. Equivalent to Rv1011,
                     len: 306 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 306 aa overlap). Probable ispE,
                     4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC
                     2.7.1.-), similar to others e.g. Q9K3R6|ISPE_STRCO
                     Streptomyces coelicolor (299 aa), FASTA scores: opt: 925,
                     E(): 2.7e-49, (54.5% identity in 297 overlap); etc.
                     BELONGS TO THE ISPE FAMILY. Protein product from Mb1038
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb1038 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P65179"
                     /db_xref="InterPro:IPR004424"
                     /db_xref="InterPro:IPR006204"
                     /db_xref="InterPro:IPR013750"
                     /db_xref="InterPro:IPR014721"
                     /db_xref="InterPro:IPR020568"
                     /db_xref="InterPro:IPR036554"
                     /db_xref="UniProtKB/Swiss-Prot:P65179"
BEGIN
        1 MPTGSVTVRV PGKVNLYLAV GDRREDGYHE LTTVFHAVSL VDEVTVRNAD VLSLELVGEG
       61 ADQLPTDERN LAWQAAELMA EHVGRAPDVS IMIDKSIPVA GGMAGGSADA AAVLVAMNSL
      121 WELNVPRRDL RMLAARLGSD VPFALHGGTA LGTGRGEELA TVLSRNTFHW VLAFADSGLL
      181 TSAVYNELDR LREVGDPPRL GEPGPVLAAL AAGDPDQLAP LLGNEMQAAA VSLDPALARA
      241 LRAGVEAGAL AGIVSGSGPT CAFLCTSASS AIDVGAQLSG AGVCRTVRVA TGPVPGARVV
      301 SAPTEV
//