LOCUS SIT99634.1 519 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 methionyl- trna synthetase mets (metrs) (methionine--trna ligase) protein. ACCESSION LT708304-1046 PROTEIN_ID SIT99634.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="metS" /locus_tag="BQ2027_MB1034C" /note="Mb1034c, metS, len: 519 aa. Equivalent to Rv1007c, len: 519 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 519 aa overlap). Probable metS (MetG), methionyl-tRNA synthetase (EC 6.1.1.10), similar to many e.g. SYM_BACSU|P37465 methionyl-tRNA synthetase from Bacillus subtilus (664 aa), FASTA scores: opt: 1506, E(): 0, (44.9% identity in 492 aa overlap); similar to other M. tuberculosis tRNA synthases e.g. Rv2448c, Rv1536, Rv0041. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. BELONGS TO CLASS-I AMINOACYL-TRNA SYNTHETASE FAMILY. STRONG, TO CYSTEINYL-TRNA SYNTHETASE. Protein product from Mb1034c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1034c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P59952" /db_xref="InterPro:IPR001412" /db_xref="InterPro:IPR009080" /db_xref="InterPro:IPR014729" /db_xref="InterPro:IPR014758" /db_xref="InterPro:IPR015413" /db_xref="InterPro:IPR023457" /db_xref="InterPro:IPR033911" /db_xref="InterPro:IPR041872" /db_xref="UniProtKB/Swiss-Prot:P59952" BEGIN 1 MKPYYVTTAI AYPNAAPHVG HAYEYIATDA IARFKRLDGY DVRFLTGTDE HGLKVAQAAA 61 AAGVPTAALA RRNSDVFQRM QEALNISFDR FIRTTDADHH EASKELWRRM SAAGDIYLDN 121 YSGWYSVRDE RFFVESETQL VDGTRLTVET GTPVTWTEEQ TYFFRLSAYT DKLLAHYHAN 181 PDFIAPETRR NEVISFVSGG LDDLSISRTS FDWGVQVPEH PDHVMYVWVD ALTNYLTGAG 241 FPDTDSELFR RYWPADLHMI GKDIIRFHAV YWPAFLMSAG IELPRRIFAH GFLHNRGEKM 301 SKSVGNIVDP VALAEALGVD QVRYFLLREV PFGQDGSYSD EAIVTRINTD LANELGNLAQ 361 RSLSMVAKNL DGRVPNPGEF ADADAALLAT ADGLLERVRG HFDAQAMHLA LEAIWLMLGD 421 ANKYFSVQQP WVLRKSESEA DQARFRTTLY VTCEVVRIAA LLIQPVMPES AGKILDLLGQ 481 APNQRSFAAV GVRLTPGTAL PPPTGVFPRY QPPQPPEGK //