LOCUS SIT99619.1 306 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 utp- -glucose-1-phosphate uridylyltransferase galu (udp-glucose pyrophosphorylase) (udpgp) (alpha-d-glucosyl-1-phosphate uridylyltransferase) (uridine diphosphoglucose pyrophosphorylase) protein. ACCESSION LT708304-1031 PROTEIN_ID SIT99619.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="galU" /locus_tag="BQ2027_MB1020" /note="Mb1020, galU, len: 306 aa. Equivalent to Rv0993, len: 306 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 306 aa overlap). Probable galU, UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), equivalent to AL035500|MLCL373_22 putative UTP-glucose-1-phosphate uridylyltransferase from Mycobacterium leprae (306 aa), FASTA score: (89.7% identity in 302 aa overlap). Also highly similar to others e.g. AB59678.1|AL132674 UTP-glucose-1-phosphate uridylyltransferase from Streptomyces coelicolor (303 aa); NP_244519.1|NC_002570 UTP-glucose-1-phosphate uridylyltransferase from Bacillus halodurans (297 aa); P25520|GALU_ECOLI|B1236|Z2012|ECS17 UTP--glucose-1-phosphate uridylyltransferase from Escherichia coli strains K12 and O157:H7 (301 aa), FASTA scores: opt: 624, E(): 2.4e-33, (38.8% identity in 299 aa overlap); etc. BELONGS TO THE PROKARYOTIC UDPGP FAMILY. Protein product from Mb1020 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1020 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XX25" /db_xref="InterPro:IPR005771" /db_xref="InterPro:IPR005835" /db_xref="InterPro:IPR029044" /db_xref="UniProtKB/TrEMBL:A0A1R3XX25" BEGIN 1 MSRPEVLTPF TAIVPAAGLG TRFLPATKTV PKELLPVVDT PGIELVAAEA AAAGAERLVI 61 VTSEGKDGVV AHFVEDLVLE GTLEARGKIA MLAKVRRAPA LIKVESVVQA EPLGLGHAIG 121 CVEPTLSPDE DAVAVLLPDD LVLPTGVLET MSKVRASRGG TVLCAIEVAR EEISAYGVFD 181 VEPVPDGDYT DDPNVLKVRG MVEKPKAETA PSRYAAAGRY VLDRAIFDAL RRIDRGAGGE 241 VQLTDAIALL IAEGHPVHVV VHQGSRHDLG NPGGYLKAAV DFALDRDDYG PDLRRWLVAR 301 LGLTEQ //