LOCUS       SIT99610.1               151 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            LARGE-CONDUCTANCE ION MECHANOSENSITIVE CHANNEL MSCL protein.
ACCESSION   LT708304-1022
PROTEIN_ID  SIT99610.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="mscL"
                     /locus_tag="BQ2027_MB1011C"
                     /note="Mb1011c, mscL, len: 151 aa. Equivalent to Rv0985c,
                     len: 151 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 151 aa overlap). Possible mscL, large
                     conductance mechanosensitive ion channel (integral
                     membrane protein), equivalent to
                     AL035500|MLCL373_19|NP_301254.1|NC_002677 putative
                     mechanosensitive channel protein from Mycobacterium leprae
                     (154 aa), FASTA score: (71.0% identity in 155 aa overlap).
                     Also highly similar to others e.g. NP_268999.1|NC_002737
                     putative large conductance mechanosensitive channel from
                     Streptococcus pyogenes (120 aa); CAB90974.1|AL355832
                     putative mechanosensitive channel from Streptomyces
                     coelicolor (156 aa); Q9X722|MSCL_CLOHI LARGE-CONDUCTANCE
                     MECHANOSENSITIVE CHANNEL from Clostridium histolyticum
                     (133 aa); Z83337|BSZ83337_6 large conductance
                     mechanosensitive channel from Bacillus subtilis (130 aa),
                     FASTA scores: opt: 248, E(): 8.4e-10, (39.0% identity in
                     136 aa overlap); U08371|ECU08371_1 large conductance
                     mechanosensitive channel from Escherichia coli strain K-12
                     (136 aa), FASTA score: (36.6% identity in 134 aa overlap);
                     etc. BELONGS TO THE MSCL FAMILY. Protein product from
                     Mb1011c detected using shotgun mass spectrometry and SWATH
                     mass spectrometry. Mb1011c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P0A5K9"
                     /db_xref="InterPro:IPR001185"
                     /db_xref="InterPro:IPR019823"
                     /db_xref="InterPro:IPR036019"
                     /db_xref="InterPro:IPR037673"
                     /db_xref="UniProtKB/Swiss-Prot:P0A5K9"
BEGIN
        1 MLKGFKEFLA RGNIVDLAVA VVIGTAFTAL VTKFTDSIIT PLINRIGVNA QSDVGILRIG
       61 IGGGQTIDLN VLLSAAINFF LIAFAVYFLV VLPYNTLRKK GEVEQPGDTQ VVLLTEIRDL
      121 LAQTNGDSPG RHGGRGTPSP TDGPRASTES Q
//