LOCUS SIT99609.1 181 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE MOAB2 (PHS) ( 4-ALPHA-HYDROXY-TETRAHYDROPTERIN DEHYDRATASE) (PTERIN- 4-A-CARBINOLAMINE DEHYDRATASE) (PHENYLALANINE HYDROXYLASE- STIMULATING PROTEIN) (PHS) (PTERIN CARBINOLAMINE DEHYDRATASE) (PCD) protein. ACCESSION LT708304-1021 PROTEIN_ID SIT99609.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="moaB2" /locus_tag="BQ2027_MB1010" /note="Mb1010, moaB2, len: 181 aa. Equivalent to Rv0984, len: 181 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 181 aa overlap). Possible moaB2, pterin-4-alpha-carbinolamine dehydratase (EC 4.2.1.96), highly similar to NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa), FASTA score: (92.3% identity in 181 aa overlap). Also similar to others e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); Q56208|MOCB_SYNP7 MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN CB from Synechococcus sp. (319 aa), FASTA score: (37.3% identity in 142 aa overlap); C-terminus of NP_197599.1|NC_003076 MOLYBDOPTERIN BIOSYNTHESIS CNX1 PROTEIN from Arabidopsis thaliana (670 aa); etc. Also similar to Rv0865|MOG from Mycobacterium tuberculosis (160 aa); and other mog proteins e.g. CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa). COULD BELONG TO THE PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE FAMILY. Alternative start codon has been suggested in position 1100508. Protein product from Mb1010 detected using shotgun mass spectrometry. Mb1010 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR001453" /db_xref="InterPro:IPR036425" /db_xref="UniProtKB/TrEMBL:A0A1R3XX15" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MKVAAQCSKL GYTVAPMEQR AELVVGRALV VVVDDRTAHG DEDHSGPLVT ELLTEAGFVV 61 DGVVAVSADE VEIRNALNTA VIGGVDLVVS VGGTGVTPRD VTPEATRDIL DREILGIAEA 121 IRASGLSAGI VDAGLSRGLA GVSGSTLVVN LAGSRYAVRD GMATLNPLAA QIIGQLSSLE 181 I //