LOCUS SIT99595.1 269 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ENOYL-COA HYDRATASE ECHA7 (ENOYL HYDRASE) (UNSATURATED ACYL-COA HYDRATASE) (CROTONASE) protein. ACCESSION LT708304-1007 PROTEIN_ID SIT99595.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="echA7" /locus_tag="BQ2027_MB0996C" /note="Mb0996c, echA7, len: 269 aa. Equivalent to Rv0971c, len: 269 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 269 aa overlap). Probable echA7, enoyl-CoA hydratase (EC 4.2.1.17), similar to many e.g. CAB95895.1|AL359988 putative enoyl CoA hydratase from Streptomyces coelicolor (247 aa); P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (257 aa), FASTA scores: opt: 369, E(): 2.6e-15, (33.7% identity in 246 aa overlap); etc. Protein product from Mb0996c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0996c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXB6" /db_xref="InterPro:IPR001753" /db_xref="InterPro:IPR014748" /db_xref="InterPro:IPR029045" /db_xref="UniProtKB/TrEMBL:A0A1R3XXB6" BEGIN 1 MDSPVDYAGP AACGGPFARL TLNSPHNRNA LSSTLVSQLH QGLSAAEADP AVRLVVLGHT 61 GGTFCAGADL SEAGGGGGDP YRMAVARARE MTALLRAIVE SPLPVVGAIN GHVRAGGFGL 121 VGACDMVVAG PESTFALTEA RIGVAPAIIS LTLLPKLSPR AAARYYLTGE KFGAREAADI 181 GLITMAADDV DAAVAALVAD VGRGSPQGLA ASKALTTAAV LEGFDRDAER LTEESARLFV 241 SDEAREGMLA FLQKRPPRWV QPATMRAAD //