LOCUS       SIT99595.1               269 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ENOYL-COA HYDRATASE ECHA7 (ENOYL HYDRASE) (UNSATURATED
            ACYL-COA HYDRATASE) (CROTONASE) protein.
ACCESSION   LT708304-1007
PROTEIN_ID  SIT99595.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="echA7"
                     /locus_tag="BQ2027_MB0996C"
                     /note="Mb0996c, echA7, len: 269 aa. Equivalent to Rv0971c,
                     len: 269 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 269 aa overlap). Probable echA7,
                     enoyl-CoA hydratase (EC 4.2.1.17), similar to many e.g.
                     CAB95895.1|AL359988 putative enoyl CoA hydratase from
                     Streptomyces coelicolor (247 aa); P24162|ECHH_RHOCA
                     enoyl-CoA hydratase from Rhodobacter capsulatus (257 aa),
                     FASTA scores: opt: 369, E(): 2.6e-15, (33.7% identity in
                     246 aa overlap); etc. Protein product from Mb0996c
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb0996c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3XXB6"
                     /db_xref="InterPro:IPR001753"
                     /db_xref="InterPro:IPR014748"
                     /db_xref="InterPro:IPR029045"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XXB6"
BEGIN
        1 MDSPVDYAGP AACGGPFARL TLNSPHNRNA LSSTLVSQLH QGLSAAEADP AVRLVVLGHT
       61 GGTFCAGADL SEAGGGGGDP YRMAVARARE MTALLRAIVE SPLPVVGAIN GHVRAGGFGL
      121 VGACDMVVAG PESTFALTEA RIGVAPAIIS LTLLPKLSPR AAARYYLTGE KFGAREAADI
      181 GLITMAADDV DAAVAALVAD VGRGSPQGLA ASKALTTAAV LEGFDRDAER LTEESARLFV
      241 SDEAREGMLA FLQKRPPRWV QPATMRAAD
//