LOCUS SIT99569.1 553 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE GLUCOSE-6-PHOSPHATE ISOMERASE PGI (GPI) (PHOSPHOGLUCOSE ISOMERASE) (PHOSPHOHEXOSE ISOMERASE) (PHI) protein. ACCESSION LT708304-981 PROTEIN_ID SIT99569.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pgi" /locus_tag="BQ2027_MB0971C" /note="Mb0971c, pgi, len: 553 aa. Equivalent to Rv0946c, len: 553 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 553 aa overlap). Probable pgi, glucose-6-phosphate isomerase (EC 5.3.1.9), equivalent to NP_301236.1|NC_002677 glucose-6-phosphate isomerase from Mycobacterium leprae (554 aa); and P96803|G6PI_MYCSM GLUCOSE-6-PHOSPHATE ISOMERASE from Mycobacterium smegmatis (442 aa). Also highly similar to others e.g. T36015 glucose-6-phosphate isomerase from Streptomyces coelicolor (551 aa); P11537|G6PI_ECOLI|GPI glucose-6-phosphate isomerase from Escherichia coli strains K12 and O157:H7 (549 aa), FASTA scores: opt: 1779, E(): 0, (51.4% identity in 554 aa overlap); etc. Contains PS00765 Phosphoglucose isomerase signature 1, and PS00174 Phosphoglucose isomerase signature 2. BELONGS TO THE GPI FAMILY. Protein product from Mb0971c detected using shotgun mass spectrometry. Mb0971c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P64193" /db_xref="InterPro:IPR001672" /db_xref="InterPro:IPR018189" /db_xref="InterPro:IPR023096" /db_xref="InterPro:IPR035476" /db_xref="InterPro:IPR035482" /db_xref="UniProtKB/Swiss-Prot:P64193" BEGIN 1 MTSAPIPDIT ATPAWDALRR HHDQIGNTHL RQFFADDPGR GRELTVSVGD LYIDYSKHRV 61 TRETLALLID LARTAHLEER RDQMFAGVHI NTSEDRAVLH TALRLPRDAE LVVDGQDVVT 121 DVHAVLDAMG AFTDRLRSGE WTGATGKRIS TVVNIGIGGS DLGPVMVYQA LRHYADAGIS 181 ARFVSNVDPA DLIATLADLD PATTLFIVAS KTFSTLETLT NATAARRWLT DALGDAAVSR 241 HFVAVSTNKR LVDDFGINTD NMFGFWDWVG GRYSVDSAIG LSLMTVIGRD AFADFLAGFH 301 IIDRHFATAP LESNAPVLLG LIGLWYSNFF GAQSRTVLPY SNDLSRFPAY LQQLTMESNG 361 KSTRADGSPV SADTGEIFWG EPGTNGQHAF YQLLHQGTRL VPADFIGFAQ PLDDLPTAEG 421 TGSMHDLLMS NFFAQTQVLA FGKTAEEIAA DGTPAHVVAH KVMPGNRPST SILASRLTPS 481 VLGQLIALYE HQVFTEGVVW GIDSFDQWGV ELGKTQAKAL LPVITGAGSP PPQSDSSTDG 541 LVRRYRTERG RAG //