LOCUS SIT99554.1 370 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PERIPLASMIC PHOSPHATE-BINDING LIPOPROTEIN PSTS2 (PBP-2) (PSTS2) protein. ACCESSION LT708304-966 PROTEIN_ID SIT99554.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pstS2" /locus_tag="BQ2027_MB0956C" /note="Mb0956c, pstS2, len: 370 aa. Equivalent to Rv0932c, len: 370 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 370 aa overlap). pstS2, phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa); Rv0928|MTCY21C12.22|pstS3 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 1601, E(): 0, (64.5% identity in 372 aa overlap); and Rv0934|MTCY08D9.05c|pstS1 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN from Mycobacterium tuberculosis (374 aa) (M. tuberculosis seems to have three PstS-like proteins, others being Rv0928 and Rv0934c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU PROTEIN ANTIGEN B PRECURSOR from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 460, E(): 2.7e-20, (31.2% identity in 375 aa overlap). Contains prokaryotic membrane lipoprotein lipid attachment site (PS00013) at N-terminus so the leader peptide of 22 aa is probably removed. BELONGS TO FAMILY OF PHOSPHATE RECEPTORS FOR BACTERIAL ABC-TYPE LIPOPROTEIN TRANSPORTERS. Appears to be co-transcribed with Rv0931c|pknD|mbk. Protein product from Mb0956c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0956c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWW7" /db_xref="InterPro:IPR005673" /db_xref="InterPro:IPR024370" /db_xref="UniProtKB/TrEMBL:A0A1R3XWW7" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MKFARSGAAV SLLAAGTLVL TACGGGTNSS SSGAGGTSGS VHCGGKKELH SSGSTAQENA 61 MEQFVYAYVR SCPGYTLDYN ANGSGAGVTQ FLNNETDFAG SDVPLNPSTG QPDRAAERCG 121 SPAWDLPTVF GPIAITYNIK GVSTLNLDGP TTAKIFNGTI TVWNDPQIQA LNSGTDLPPT 181 PISVIFRSDK SGTSDNFQKY LDGASNGAWG KGASETFNGG VGVGASGNNG TSALLQTTDG 241 SITYNEWSFA VGKQLNMAQI ITSAGPDPVA ITTESVGKTI AGAKIMGQGN DLVLDTSSFY 301 RPTQPGSYPI VLATYEIVCS KYPDATTGTA VRAFMQAAIG PGQEGLDQYG SIPLPKSFQA 361 KLAAAVNAIS //