LOCUS       SIT99552.1               369 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 TRANSMEMBRANE
            SERINE/THREONINE-PROTEIN KINASE D PKNDb [SECOND PART](
            PROTEIN KINASE D) (STPK D) protein.
ACCESSION   LT708304-964
PROTEIN_ID  SIT99552.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="pknDb"
                     /locus_tag="BQ2027_MB0954C"
                     /standard_name="mbk"
                     /note="Mb0954c, pknDb, len: 369 aa. Equivalent to 3' end
                     of Rv0931c, len: 664 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (100.0% identity in 369 aa overlap). pknD
                     (alternate gene name: mbk), transmembrane serine/threonine
                     protein kinase (EC 2.7.1.-) (see citations below),
                     equivalent to CAB62227.1|AJ250200 putative
                     serine/threonine protein kinase from Mycobacterium bovis
                     BCG (291 aa); and highly similar in N-terminus to
                     P54744|PKNB_MYCLE probable serine/threonine-specific
                     protein kinase (EC 2.7.1.-) from Mycobacterium leprae (622
                     aa). Also highly similar to others, particularly in
                     N-terminal half e.g. NP_243370.1|NC_002570
                     serine/threonine protein kinase from Bacillus halodurans
                     (664 aa); NP_268044.1|NC_002662 serine/threonine protein
                     kinase from Lactococcus lactis (627 aa); etc. Also highly
                     similar to other serine/threonine protein kinases from
                     Mycobacterium tuberculosis e.g. pknH (626 aa), FASTA
                     scores: opt: 1398, E: 0, (49.3% identity in 540 aa
                     overlap); pknE (566 aa); pknB (626 aa); Rv3524 (343 aa);
                     etc. Contains Hank's kinase subdomain. Contains two
                     transmembrane segments, which flank a highly repetitive
                     region, suggesting a receptor-like anchoring. BELONGS TO
                     THE SER/THR FAMILY OF PROTEIN KINASES. Experimental
                     studies show evidence of auto-phosphorylation on a serine
                     residue. Appears to be co-transcribed with Rv0932c|pstS2.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, pknD exists as a single gene.
                     In Mycobacterium bovis, a frameshift due to a single base
                     insertion (*-t) splits pknD into 2 parts, pknDa and pknDb.
                     Protein product from Mb0954c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb0954c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3XYY6"
                     /db_xref="InterPro:IPR001258"
                     /db_xref="InterPro:IPR011042"
                     /db_xref="InterPro:IPR013017"
                     /db_xref="InterPro:IPR035016"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XYY6"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MLATPADTGL SQSESGIAGA GTGPPTPGAA RWSPGDSATV AGPLAADSRG GNWPSQTGHS
       61 PAVPNALQAS LGHAVPPAGN KRKVWAVVGA AAIVLVAIVA AAGYLVLRPS WSPTQASGQT
      121 VLPFTGIDFR LSPSGVAVDS AGNVYVTSEG MYGRVVKLAT GSTGTTVLPF NGLYQPQGLA
      181 VDGAGTVYVT DFNNRVVTLA AGSNNQTVLP FDGLNYPEGL AVDTQGAVYV ADRGNNRVVK
      241 LAAGSKTQTV LPFTGLNDPD GVAVDNSGNV YVTDTDNNRV VKLEAESNNQ VVLPFTDITA
      301 PWGIAVDEAG TVYVTEHNTN QVVKLLAGST TSTVLPFTGL NTPLAVAVDS DRTVYVADRG
      361 NDRVVKLTS
//