LOCUS SIT99552.1 369 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 TRANSMEMBRANE SERINE/THREONINE-PROTEIN KINASE D PKNDb [SECOND PART]( PROTEIN KINASE D) (STPK D) protein. ACCESSION LT708304-964 PROTEIN_ID SIT99552.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pknDb" /locus_tag="BQ2027_MB0954C" /standard_name="mbk" /note="Mb0954c, pknDb, len: 369 aa. Equivalent to 3' end of Rv0931c, len: 664 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 369 aa overlap). pknD (alternate gene name: mbk), transmembrane serine/threonine protein kinase (EC 2.7.1.-) (see citations below), equivalent to CAB62227.1|AJ250200 putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa); and highly similar in N-terminus to P54744|PKNB_MYCLE probable serine/threonine-specific protein kinase (EC 2.7.1.-) from Mycobacterium leprae (622 aa). Also highly similar to others, particularly in N-terminal half e.g. NP_243370.1|NC_002570 serine/threonine protein kinase from Bacillus halodurans (664 aa); NP_268044.1|NC_002662 serine/threonine protein kinase from Lactococcus lactis (627 aa); etc. Also highly similar to other serine/threonine protein kinases from Mycobacterium tuberculosis e.g. pknH (626 aa), FASTA scores: opt: 1398, E: 0, (49.3% identity in 540 aa overlap); pknE (566 aa); pknB (626 aa); Rv3524 (343 aa); etc. Contains Hank's kinase subdomain. Contains two transmembrane segments, which flank a highly repetitive region, suggesting a receptor-like anchoring. BELONGS TO THE SER/THR FAMILY OF PROTEIN KINASES. Experimental studies show evidence of auto-phosphorylation on a serine residue. Appears to be co-transcribed with Rv0932c|pstS2. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, pknD exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base insertion (*-t) splits pknD into 2 parts, pknDa and pknDb. Protein product from Mb0954c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0954c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XYY6" /db_xref="InterPro:IPR001258" /db_xref="InterPro:IPR011042" /db_xref="InterPro:IPR013017" /db_xref="InterPro:IPR035016" /db_xref="UniProtKB/TrEMBL:A0A1R3XYY6" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MLATPADTGL SQSESGIAGA GTGPPTPGAA RWSPGDSATV AGPLAADSRG GNWPSQTGHS 61 PAVPNALQAS LGHAVPPAGN KRKVWAVVGA AAIVLVAIVA AAGYLVLRPS WSPTQASGQT 121 VLPFTGIDFR LSPSGVAVDS AGNVYVTSEG MYGRVVKLAT GSTGTTVLPF NGLYQPQGLA 181 VDGAGTVYVT DFNNRVVTLA AGSNNQTVLP FDGLNYPEGL AVDTQGAVYV ADRGNNRVVK 241 LAAGSKTQTV LPFTGLNDPD GVAVDNSGNV YVTDTDNNRV VKLEAESNNQ VVLPFTDITA 301 PWGIAVDEAG TVYVTEHNTN QVVKLLAGST TSTVLPFTGL NTPLAVAVDS DRTVYVADRG 361 NDRVVKLTS //