LOCUS SIT99548.1 263 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable short-chain type dehydrogenase/reductase protein. ACCESSION LT708304-960 PROTEIN_ID SIT99548.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0950C" /note="Mb0950c, -, len: 263 aa. Equivalent to Rv0927c, len: 263 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 263 aa overlap). Probable short-chain dehydrogenase/reductase (EC 1.-.-.-), similar to various dehydrogenases/reductases, notably 7-alpha-hydroxysteroid dehydrogenases and glucose 1-dehydrogenases e.g. P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 551, E(): 1e-26, (39.5% identity in 248 aa overlap); NP_252778.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Also similar to other dehydrogenases from Mycobacterium tuberculosis e.g. MTCY09F9.36, E():1.4e-18; MTCY369.14, E():8e-17; MTCY02B10.14, E():2.5e-14; MTCY09F9.23c, E():1.5e-13; MTCY03C7.07, E():1.9e-13. Contains PS00061 Short-chain dehydrogenases/reductases family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). BELONGS TO THE SHORT-CHAIN DEHYDROGENASES/REDUCTASES (SDR) FAMILY. Protein product from Mb0950c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0950c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWT1" /db_xref="InterPro:IPR002347" /db_xref="InterPro:IPR020904" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3XWT1" BEGIN 1 MILDMFRLDD KVAVITGGGR GLGAAIALAF AQAGADVLIA SRTSSELDAV AEQIRAAGRR 61 AHTVAADLAH PEVTAQLAGQ AVGAFGKLDI VVNNVGGTMP NTLLSTSTKD LADAFAFNVG 121 TAHALTVAAV PLMLEHSGGG SVINISSTMG RLAARGFAAY GTAKAALAHY TRLAALDLCP 181 RVRVNAIAPG SILTSALEVV AANDELRAPM EQATPLRRLG DPVDIAAAAV YLASPAGSFL 241 TGKTLEVDGG LTFPNLDLPI PDL //