LOCUS SIT99524.1 446 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 TWO COMPONENT SENSOR HISTIDINE KINASE PRRB protein. ACCESSION LT708304-936 PROTEIN_ID SIT99524.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="prrB" /locus_tag="BQ2027_MB0926C" /note="Mb0926c, prrB, len: 446 aa. Equivalent to Rv0902c, len: 446 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 446 aa overlap). prrB, two-component sensor histidine kinase (EC 2.7.3.-) (see citations below), transmembrane protein, equivalent to MLCB57_26|NP_302403.1|NC_002677 sensor histidine kinase from Mycobacterium leprae (446 aa); and similar at C-termini to NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa). C-terminus also similar to the C-termini of many sensor-like histidine kinase proteins e.g. P08336|CPXA_ECOLI|ECFB|SSD|EUP|B3911|Z5456|ECS4837 sensor protein from Escherichia coli strain K12 (457 aa), FASTA scores: opt: 364, E(): 1.7e-15, (27.1% identity in 398 aa overlap); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (483 aa); CAB82845.1|AJ277081 putative histidine kinase from Amycolatopsis mediterranei (472 aa); etc. Also similar in part to Mycobacterium tuberculosis proteins Rv3764c (475 aa); and Rv0982 (504 aa). Thought to be induced at phagocytosis (see second citation below). Protein product from Mb0926c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0926c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A5Z9" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR003661" /db_xref="InterPro:IPR004358" /db_xref="InterPro:IPR005467" /db_xref="InterPro:IPR036097" /db_xref="InterPro:IPR036890" /db_xref="UniProtKB/Swiss-Prot:P0A5Z9" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNILSRIFAR TPSLRTRVVV ATAIGAAIPV LIVGTVVWVG ITNDRKERLD RRLDEAAGFA 61 IPFVPRGLDE IPRSPNDQDA LITVRRGNVI KSNSDITLPK LQDDYADTYV RGVRYRVRTV 121 EIPGPEPTSV AVGATYDATV AETNNLHRRV LLICTFAIGA AAVFAWLLAA FAVRPFKQLA 181 EQTRSIDAGD EAPRVEVHGA SEAIEIAEAM RGMLQRIWNE QNRTKEALAS ARDFAAVSSH 241 ELRTPLTAMR TNLEVLSTLD LPDDQRKEVL NDVIRTQSRI EATLSALERL AQGELSTSDD 301 HVPVDITDLL DRAAHDAARI YPDLDVSLVP SPTCIIVGLP AGLRLAVDNA IANAVKHGGA 361 TLVQLSAVSS RAGVEIAIDD NGSGVPEGER QVVFERFSRG STASHSGSGL GLALVAQQAQ 421 LHGGTASLEN SPLGGARLVL RLPGPS //