LOCUS SIT99511.1 373 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CITRATE SYNTHASE II CITA protein. ACCESSION LT708304-923 PROTEIN_ID SIT99511.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="citA" /locus_tag="BQ2027_MB0913C" /note="Mb0913c, citA, len: 373 aa. Equivalent to Rv0889c, len: 373 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 373 aa overlap). Probable citA (alternate gene name: gltA), citrate synthase 2 (EC 4.1.3.7), highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30, (33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. BELONGS TO THE CITRATE SYNTHASE FAMILY. Protein product from Mb0913c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0913c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P63778" /db_xref="InterPro:IPR002020" /db_xref="InterPro:IPR016142" /db_xref="InterPro:IPR016143" /db_xref="InterPro:IPR019810" /db_xref="InterPro:IPR036969" /db_xref="UniProtKB/Swiss-Prot:P63778" BEGIN 1 MTVVPENFVP GLDGVVAFTT EIAEPDKDGG ALRYRGVDIE DLVSQRVTFG DVWALLVDGN 61 FGSGLPPAEP FPLPIHSGDV RVDVQAGLAM LAPIWGYAPL LDIDDATARQ QLARASVMAL 121 SYVAQSARGI YQPAVPQRII DECSTVTARF MTRWQGEPDP RHIEAIDAYW VSAAEHGMNA 181 STFTARVIAS TGADVAAALS GAIGAMSGPL HGGAPARVLP MLDEVERAGD ARSVVKGILD 241 RGEKLMGFGH RVYRAEDPRA RVLRAAAERL GAPRYEVAVA VEQAALSELR ERRPDRAIET 301 NVEFWAAVVL DFARVPANMM PAMFTCGRTA GWCAHILEQK RLGKLVRPSA IYVGPGPRSP 361 ESVDGWERVL TTA //