LOCUS SIT99481.1 403 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE ACYL-COA THIOLASE FADA protein. ACCESSION LT708304-893 PROTEIN_ID SIT99481.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadA" /locus_tag="BQ2027_MB0882" /note="Mb0882, fadA, len: 403 aa. Equivalent to Rv0859, len: 403 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 403 aa overlap). Possible fadA, acyl-CoA thiolase (EC 2.3.1.-), equivalent to NP_302423.1|NC_002677 putative beta-ketoadipyl CoA thiolase from Mycobacterium leprae (403 aa). Also highly similar to acyl/acetyl-CoA thiolases and beta-ketoadipyl CoA thiolases, e.g. T35428 probable acetyl CoA acetyltransferase (thiolase) from Streptomyces coelicolor (404 aa); NP_250427.1|NC_002516 probable acyl-CoA thiolase from Pseudomonas aeruginosa (401 aa); NP_106253.1|NC_002678 probable acyl-CoA thiolase from Mesorhizobium loti (402 aa); NP_248919.1|NC_002516|PcaF beta-ketoadipyl CoA thiolase PcaF from Pseudomonas aeruginosa (401 aa); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2 and PS00099 Thiolases active site. Protein product from Mb0882 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0882 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWM6" /db_xref="InterPro:IPR002155" /db_xref="InterPro:IPR016039" /db_xref="InterPro:IPR020610" /db_xref="InterPro:IPR020613" /db_xref="InterPro:IPR020615" /db_xref="InterPro:IPR020616" /db_xref="InterPro:IPR020617" /db_xref="UniProtKB/TrEMBL:A0A1R3XWM6" BEGIN 1 MSEEAFIYEA IRTPRGKQKN GSLHEVKPLS LVVGLIDELR KRHPDLDENL ISDVILGCVS 61 PVGDQGGDIA RAAVLASGMP VTSGGVQLNR FCASGLEAVN TAAQKVRSGW DDLVLAGGVE 121 SMSRVPMGSD GGAMGLDPAT NYDVMFVPQG IGADLIATIE GFSREDVDAY ALRSQQKAAE 181 AWSGGYFAKS VVPVRDQNGL LILDHDEHMR PDTTKEGLAK LKPAFEGLAA LGGFDDVALQ 241 KYHWVEKINH VHTGGNSSGI VDGAALVMIG SAAAGKLQGL TPRARIVATA TSGADPVIML 301 TGPTPATRKV LDRAGLTVDD IDLFELNEAF ASVVLKFQKD LNIPDEKLNV NGGAIAMGHP 361 LGATGAMILG TMVDELERRN ARRALITLCI GGGMGVATII ERV //