LOCUS SIT99475.1 560 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PYRUVATE OR INDOLE-3-PYRUVATE DECARBOXYLASE PDC protein. ACCESSION LT708304-887 PROTEIN_ID SIT99475.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pdc" /locus_tag="BQ2027_MB0876C" /note="Mb0876c, pdc, len: 560 aa. Equivalent to Rv0853c, len: 560 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 560 aa overlap). Probable pdc, pyruvate or indole-pyruvate decarboxylase (EC 4.1.1.-), equivalent to NP_302424.1|NC_002677 pyruvate (or indolepyruvate) decarboxylase from Mycobacterium leprae (569 aa). Also highly similar to others e.g. AAB06571.1|L80006 indolepyruvate decarboxylase from Pantoea agglomerans (550 aa); Q12629|DCPY_KLULA PYRUVATE DECARBOXYLASE (EC 4.1.1.1) from Kluyveromyces marxianus var. lactis (563 aa); P71323 INDOLEPYRUVATE DECARBOXYLASE (EC 4.1.1.74) from Enterobacter herbicola (550 aa), FASTA scores: opt: 1642, E(): 0, (48.1% identity in 547 aa overlap); P23234|DCIP_ENTCL INDOLE-3-PYRUVATE DECARBOXYLASE (INDOLEPYRUVATE DECARBOXYLASE) from Enterobacter cloacae (552 aa), FASTA scores: opt: 1596, E(): 0, (46.8% identity in 551 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature and PS00017 ATP/GTP-binding site motif A (P-loop). COFACTOR: THIAMINE PYROPHOSPHATE. Protein product from Mb0876c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0876c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7U140" /db_xref="InterPro:IPR000399" /db_xref="InterPro:IPR011766" /db_xref="InterPro:IPR012000" /db_xref="InterPro:IPR012001" /db_xref="InterPro:IPR012110" /db_xref="InterPro:IPR029035" /db_xref="InterPro:IPR029061" /db_xref="UniProtKB/Swiss-Prot:Q7U140" BEGIN 1 MTPQKSDACS DPVYTVGDYL LDRLAELGVS EIFGVPGDYN LQFLDHIVAH PTIRWVGSAN 61 ELNAGYAADG YGRLRGMSAV VTTFGVGELS VTNAIAGSYA EHVPVVHIVG GPTKDAQGTR 121 RALHHSLGDG DFEHFLRISR EITCAQANLM PATAGREIDR VLSEVREQKR PGYILLSSDV 181 ARFPTEPPAA PLPRYPGGTS PRALSLFTKA AIELIADHQL TVLADLLVHR LQAVKELEAL 241 LAADVVPHAT LMWGKSLLDE SSPNFLGIYA GAASAERVRA AIEGAPVLVT AGVVFTDMVS 301 GFFSQRIDPA RTIDIGQYQS SVADQVFAPL EMSAALQALA TILTGRGISS PPVVPPPAEP 361 PPAMPARDEP LTQQMVWDRV CSALTPGNVV LADQGTSFYG MADHRLPQGV TFIGQPLWGS 421 IGYTLPAAVG AAVAHPDRRT VLLIGDGAAQ LTVQELGTFS REGLSPVIVV VNNDGYTVER 481 AIHGETAPYN DIVSWNWTEL PSALGVTNHL AFRAQTYGQL DDALTVAAAR RDRMVLVEVV 541 LPRLEIPRLL GQLVGSMAPQ //